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EC 6.3.2.50 Details
show Brenda entryEC number
6.3.2.50
Accepted name
tenuazonic acid synthetase
Reaction
ATP + L-isoleucine + acetoacetyl-CoA = AMP + diphosphate + tenuazonic acid + CoA
Other name(s)
TAS1 (gene name)
Systematic name
L-isoleucine:acetoacetyl-CoA ligase (tenuazonic acid-forming)
Comment
This fungal enzyme, isolated from Magnaporthe oryzae, is an non-ribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) hybrid protein that consists of condensation (C), adenylation (A) and peptidyl-carrier protein (PCP) domains in the NRPS portion and a ketosynthase (KS) domain in the PKS portion. ATP is required for activation of isoleucine, which is then condensed with acetoacetyl-CoA. Cyclization and release from the enzyme are catalysed by the KS domain.
History
created 2017
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.14.2 created 1972, deleted 1976 1.14.14.4 created 2000, deleted 2002
