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EC 1.14.11.21 Details

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EC number

1.14.11.21

Accepted name

clavaminate synthase

Reaction

(1) deoxyamidinoproclavaminate + 2-oxoglutarate + O₂ = amidinoproclavaminate + succinate + CO₂;;(2) proclavaminate + 2-oxoglutarate + O₂ = dihydroclavaminate + succinate + CO₂ + H₂O;;(3) dihydroclavaminate + 2-oxoglutarate + O₂ = clavaminate + succinate + CO₂ + H₂O

Other name(s)

clavaminate synthase 2, clavaminic acid synthase

Systematic name

deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)

CAS registry number

122799-56-8

Comment

Contains nonheme iron. Catalyses three separate oxidative reactions in the pathway for the biosythesis of the β-lactamase inhibitor clavulanate in Streptomyces clavuligerus. The first step (hydroxylation) is separated from the latter two (oxidative cyclization and desaturation) by the action of EC 3.5.3.22, proclavaminate amidinohydrolase. The three reactions are all catalysed at the same nonheme iron site.

History

created 2003

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.1 With NADH or NADPH as one donor (deleted sub-subclass)

1.14.2 With ascorbate as one donor (deleted sub-subclass)

1.14.3 With reduced pteridine as one donor (deleted sub-subclass)

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.12.1 anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

1.14.12.2 created 1972, deleted 1990

1.14.12.3 benzene 1,2-dioxygenase

1.14.12.4 3-hydroxy-2-methylpyridinecarboxylate dioxygenase

1.14.12.5 5-pyridoxate dioxygenase

1.14.12.6 created 1978, deleted 1999

1.14.12.7 phthalate 4,5-dioxygenase

1.14.12.8 4-sulfobenzoate 3,4-dioxygenase

1.14.12.9 4-chlorophenylacetate 3,4-dioxygenase

1.14.12.10 benzoate 1,2-dioxygenase

1.14.12.11 toluene dioxygenase

1.14.12.12 naphthalene 1,2-dioxygenase

1.14.12.13 2-halobenzoate 1,2-dioxygenase

1.14.12.14 2-aminobenzenesulfonate 2,3-dioxygenase

1.14.12.15 terephthalate 1,2-dioxygenase

1.14.12.16 2-hydroxyquinoline 5,6-dioxygenase

1.14.12.17 nitric oxide dioxygenase

1.14.12.18 biphenyl 2,3-dioxygenase

1.14.12.19 3-phenylpropanoate dioxygenase

1.14.12.20 pheophorbide a oxygenase

1.14.12.21 benzoyl-CoA 2,3-dioxygenase

1.14.12.22 carbazole 1,9a-dioxygenase

1.14.12.23 nitroarene dioxygenase

1.14.12.24 2,4-dinitrotoluene dioxygenase

1.14.12.25 p-cumate 2,3-dioxygenase

1.14.12.26 chlorobenzene dioxygenase

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor

1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.21 With NADH or NADPH as one donor, and the other dehydrogenated

1.14.99 Miscellaneous

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

3.1 Acting on ester bonds

3.2 Glycosylases

3.3 Acting on ether bonds

3.4 Acting on peptide bonds (peptidases)

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.6 Acting on acid anhydrides

3.7 Acting on carbon-carbon bonds

3.8 Acting on halide bonds

3.9 Acting on phosphorus-nitrogen bonds

3.10 Acting on sulfur-nitrogen bonds

3.10.1 Acting on sulfur-nitrogen bonds (only sub-subclass identified to date)

3.11 Acting on carbon-phosphorus bonds

3.12 Acting on sulfur-sulfur bonds

3.13 Acting on carbon-sulfur bonds

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Release 2023.1 (January 2023)