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EC 1.14.14.3 Details
show Brenda entryEC number
1.14.14.3
Accepted name
bacterial luciferase
Reaction
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hν
Other name(s)
aldehyde monooxygenase,
luciferase,
Vibrio fischeri luciferase,
alkanal,reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing),
alkanal,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing),
alkanal monooxygenase (FMN),
aldehyde,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
Systematic name
long-chain-aldehyde,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
CAS registry number
9014-00-0
Comment
The reaction sequence starts with the incorporation of a molecule of oxygen into reduced FMN bound to the enzyme, forming luciferase peroxyflavin. The peroxyflavin interacts with an aliphatic long-chain aldehyde, producing a highly fluorescent species believed to be luciferase hydroxyflavin. The enzyme is highly specific for reduced FMN and for long-chain aliphatic aldehydes with eight carbons or more. The highest efficiency is achieved with tetradecanal. cf. EC 1.13.12.18, dinoflagellate luciferase.
History
created 1981, modified 2016
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003 1.14.17.2 created 1972, deleted 1984
