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EC 6.3.1.20 Details
EC number
6.3.1.20
Accepted name
lipoate—protein ligase
Reaction
ATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N6-(lipoyl)lysine + AMP + diphosphate (overall reaction) ;;(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate;;(1b) lipoyl-AMP + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N6-(lipoyl)lysine + AMP
Other name(s)
lplA (gene name), lplJ (gene name), lipoate protein ligase, lipoate-protein ligase A, LPL, LPL-B
Systematic name
[lipoyl-carrier protein]-L-lysine:lipoate ligase (AMP-forming)
CAS registry number
144114-18-1
Comment
Requires Mg2+. This enzyme participates in lipoate salvage, and is responsible for lipoylation in the presence of exogenous lipoic acid [7]. The enzyme attaches lipoic acid to the lipoyl domains of certain key enzymes involved in oxidative metabolism, including pyruvate dehydrogenase (E2 domain), 2-oxoglutarate dehydrogenase (E2 domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein) [6]. Lipoylation is essential for the function of these enzymes. The enzyme can also use octanoate instead of lipoate.
History
created 2006 as EC 2.7.7.63, transferred 2016 to EC 6.3.1.20
EC Tree
1.14.11.5 created 1972, deleted 1976
1.14.17.2 created 1972, deleted 1984