EC Explorer

1.14.14.145

abieta-7,13-dien-18-ol hydroxylase

abieta-7,13-dien-18-ol + 2 [reduced NADPH—hemoprotein reductase] + 2 O₂ = abieta-7,13-dien-18-oate + 2 [oxidized NADPH—hemoprotein reductase] + 3 H₂O (overall reaction);;(1a) abieta-7,13-dien-18-ol + [reduced NADPH—hemoprotein reductase] + O₂ = abieta-7,13-dien-18,18-diol + [oxidized NADPH—hemoprotein reductase] + H₂O;;(1b) abieta-7,13-dien-18,18-diol = abieta-7,13-dien-18-al + H₂O (spontaneous);;(1c) abieta-7,13-dien-18-al + [reduced NADPH—hemoprotein reductase] + O₂ = abieta-7,13-dien-18-oate + [oxidized NADPH—hemoprotein reductase] + H₂O

CYP720B1, PtAO, abietadienol hydroxylase (ambiguous)

abieta-7,13-dien-18-ol,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)

A cytochrome P-450 (heme-thiolate) protein. This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine) [1], and the gene encoding the enzyme has been identified in Pinus taeda (loblolly pine) [3]. The recombinant enzyme catalyses the oxidation of multiple diterpene alcohol and aldehydes, including levopimaradienol, isopimara-7,15-dienol, isopimara-7,15-dienal, dehydroabietadienol and dehydroabietadienal. It is not able to oxidize abietadiene.

created 2009 as EC 1.14.13.109, modified 2012, transferred 2018 to EC 1.14.14.145