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EC 1.14.14.139 Details
EC number
1.14.14.139
Accepted name
5β-cholestane-3α,7α-diol 12α-hydroxylase
Reaction
(1) 5β-cholestane-3α,7α-diol + [reduced NADPH—hemoprotein reductase] + O2 = 5β-cholestane-3α,7α,12α-triol + [oxidized NADPH—hemoprotein reductase] + H2O;; (2) 7α-hydroxycholest-4-en-3-one + [reduced NADPH—hemoprotein reductase] + O2 = 7α,12α-dihydroxycholest-4-en-3-one + [oxidized NADPH—hemoprotein reductase] + H2O;; (3) chenodeoxycholate + [reduced NADPH—hemoprotein reductase] + O2 = cholate + [oxidized NADPH—hemoprotein reductase] + H2O
Other name(s)
5β-cholestane-3α,7α-diol 12α-monooxygenase, sterol 12α-hydroxylase (ambiguous), CYP8B1, cytochrome P450 8B1, 7α-hydroxycholest-4-en-3-one 12α-hydroxylase, 7α-hydroxy-4-cholesten-3-one 12α-monooxygenase, chenodeoxycholate 12&alpha, monooxygenase
Systematic name
5β-cholestane-3α,7α-diol,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (12α-hydroxylating)
Comment
A cytochrome P-450 (heme-thiolate) protein found in mammals. This is the key enzyme in the biosynthesis of the bile acid cholate. The enzyme can also hydroxylate 5β-cholestane-3α,7α-diol at the 25 and 26 position, but to a lesser extent [2].
History
created 2005 as EC 1.14.13.96, transferred 2018 to EC 1.14.14.139 (EC 1.14.18.8 created 2005 as EC 1.14.13.95, transferred 2015 to EC 1.14.18.8, incorporated 2020) , modified 2020
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