EC Explorer

1.14.13.241

5-pyridoxate monooxygenase

3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + H⁺ + O₂ = 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP⁺

5-pyridoxate,NADPH:oxygen oxidoreductase (decyclizing), 5-pyridoxate oxidase (misleading), 5-pyridoxate dioxygenase (incorrect)

5-pyridoxate,NADPH:oxygen oxidoreductase (ring-opening)

37256-70-5

Contains FAD. The enzyme, characterized from the bacterium Arthrobacter sp. Cr-7, participates in the degradation of pyridoxine (vitamin B₆). Although the enzyme was initially thought to be a dioxygenase, oxygen-tracer experiments have suggested that it is a monooxygenase, incorporating only one oxygen atom from molecular oxygen into the product. The second oxygen atom originates from a water molecule, which is regenerated during the reaction and thus does not show up in the reaction equation.

created 2018 (EC 1.14.12.5 created 1972, incorporated 2018)