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EC 6.1.2.2 Details
show Brenda entryEC number
6.1.2.2
Accepted name
nebramycin 5′ synthase
Reaction
(1) tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5′ + AMP + diphosphate + phosphate (overall reaction);;(1a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate;;(1b) O-carbamoyladenylate + tobramycin = AMP + nebramycin 5′ ;;(2) kanamycin A + carbamoyl phosphate + ATP + H2O = 6′′-O-carbamoylkanamycin A + AMP + diphosphate + phosphate (overall reaction);;(2a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate;;(2b) O-carbamoyladenylate + kanamycin A = AMP + 6′′-O-carbamoylkanamycin A
Other name(s)
tobramycin carbamoyltransferase,
TobZ
Systematic name
tobramycin:carbamoyl phosphate ligase (AMP,phosphate-forming)
Comment
Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
History
created 2014
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.19.1 stearoyl-CoA 9-desaturase
