EC Explorer

2.7.1.190

aminoglycoside 2′′-phosphotransferase

GTP + gentamicin = GDP + gentamicin 2′′-phosphate

aphD (gene name), APH(2′′), aminoglycoside (2′′) kinase, gentamicin kinase (ambiguous), gentamicin phosphotransferase (ambiguous)

GTP:gentamicin 2′′-O-phosphotransferase

Requires Mg²⁺. This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2′′, including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin. In most, but not all, cases the phosphorylation confers resistance against the antibiotic. Some forms of the enzyme use ATP as a phosphate donor in appreciable amount. The enzyme is often found as a bifunctional enzyme that also catalyses 6′-aminoglycoside N-acetyltransferase activity. The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.

created 2015