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EC 1.5.99.4 Details

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EC number

1.5.99.4

Accepted name

nicotine dehydrogenase

Reaction

(S)-nicotine + acceptor + H₂O = (S)-6-hydroxynicotine + reduced acceptor

Other name(s)

nicotine oxidase, D-nicotine oxidase, nicotine:(acceptor) 6-oxidoreductase (hydroxylating), L-nicotine oxidase

Systematic name

nicotine:acceptor 6-oxidoreductase (hydroxylating)

CAS registry number

37256-31-8

Comment

A metalloprotein (FMN). The enzyme can act on both the naturally found (S)-enantiomer and the synthetic (R)-enantiomer of nicotine, with retention of configuration in both cases [4].

History

created 1972

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.6.1 With NAD⁺ or NADP⁺ as acceptor

1.6.2 With a heme protein as acceptor

1.6.3 With oxygen as acceptor

1.6.4 With a disulfide as acceptor (deleted sub-subclass)

1.6.4.1 created 1961, deleted 2002

1.6.4.2 created 1961, modified 1989, deleted 2002

1.6.4.3 created 1961, modified 1976, deleted 1983

1.6.4.4 created 1965, deleted 2002

1.6.4.5 created 1972, deleted 2002

1.6.4.6 created 1972, deleted 2002

1.6.4.7 created 1978, deleted 2002

1.6.4.8 created 1989, deleted 2002

1.6.4.9 created 1992, deleted 2002

1.6.4.10 created 1992, deleted 2002

1.6.5 With a quinone or similar compound as acceptor

1.6.5.1 created 1961, deleted 1965

1.6.5.2 NAD(P)H dehydrogenase (quinone)

1.6.5.3 NADH:ubiquinone reductase (H⁺-translocating)

1.6.5.4 monodehydroascorbate reductase (NADH)

1.6.5.5 NADPH:quinone reductase

1.6.5.6 p-benzoquinone reductase (NADPH)

1.6.5.7 2-hydroxy-1,4-benzoquinone reductase

1.6.5.8 NADH:ubiquinone reductase (Na⁺-transporting)

1.6.5.9 NADH:quinone reductase (non-electrogenic)

1.6.5.10 NADPH dehydrogenase (quinone)

1.6.5.11 NADH dehydrogenase (quinone)

1.6.5.12 demethylphylloquinone reductase

1.6.6 With a nitrogenous group as acceptor

1.6.7 With an iron-sulfur protein as acceptor (deleted sub-subclass)

1.6.8 With a flavin as acceptor (deleted sub-subclass)

1.6.99 With unknown physiological acceptors

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.97.1 Sole sub-subclass for oxidoreductases that do not belong in the other subclasses

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)