EC Explorer
[browse EC tree] - [search]
EC 1.5.1.28 Details
show Brenda entryEC number
1.5.1.28
Accepted name
opine dehydrogenase
Reaction
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O = L-2-aminopentanoic acid + pyruvate + NADH + H+
Other name(s)
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD+, L-aminopentanoate-forming)
Systematic name
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming)
CAS registry number
108281-02-3
Comment
In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate. In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor. They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine. The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.
History
created 1999
back to topEC Tree
1 Oxidoreductases
1.1.1 With NAD+ or NADP+ as acceptor
1.1.99.15 created 1978, deleted 1980 1.1.99.17 created 1982, deleted 2003 1.5.3.3 created 1961, deleted 1972 1.5.3.8 created 1989, deleted 1992 1.5.3.9 created 1989, modified 1999, deleted 2002
