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EC 4.1.99.13 Details
EC number
4.1.99.13
Accepted name
(6-4)DNA photolyase
Reaction
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)
Other name(s)
DNA photolyase, H64PRH, NF-10, phr (6-4), PL-(6-4), OtCPF1, (6-4) PHR, At64PHR
Systematic name
(6-4) photoproduct pyrimidine-lyase
CAS registry number
37290-70-3
Comment
A flavoprotein (FAD). The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases. This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases [2].
History
created 2009
EC Tree
1.1.99.15 created 1978, deleted 1980
1.1.99.17 created 1982, deleted 2003