EC Explorer

1.8.3.7

formylglycine-generating enzyme

a [sulfatase]-L-cysteine + O₂ + 2 a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H₂O

sulfatase-modifying factor 1, Cα-formylglycine-generating enzyme 1, SUMF1 (gene name)

[sulfatase]-L-cysteine:oxygen oxidoreductase (3-oxo-L-alanine-forming)

Requires a copper cofactor and Ca²⁺. The enzyme, which is found in both prokaryotes and eukaryotes, catalyses a modification of a conserved L-cysteine residue in the active site of sulfatases, generating a unique 3-oxo-L-alanine residue that is essential for sulfatase activity. The exact nature of the thiol involved is still not clear - dithiothreitol and cysteamine are the most efficiently used thiols in vitro. Glutathione alo acts in vitro, but it is not known whether it is used in vivo.

created 2014