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EC 2.8.1.15 Details

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EC number

2.8.1.15

Accepted name

tRNA-5-methyluridine⁵⁴ 2-sulfurtransferase

Reaction

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluracil⁵⁴ in tRNA + H₂O = AMP + diphosphate + 5-methyl-2-thiouracil⁵⁴ in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly

Other name(s)

TtuA

Systematic name

[TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH:tRNA (5-methyluridine⁵⁴-2-O)-sulfurtransferase

Comment

The enzyme, found in thermophilic bacteria and archaea, modifies the ribothymidine (5-methyluridine) residue at position 54 of tRNAs. Contains zinc and an [4Fe-4S] cluster. Some organisms, such as the archaeon Pyrococcus horikoshii, do not have a TtuB sulfur-carrier protein, and appear to use sulfide as the sulfur source.

History

created 2017

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.2 With a cytochrome as acceptor

1.1.3 With oxygen as acceptor

1.1.4 With a disulfide as acceptor

1.1.5 With a quinone or similar compound as acceptor

1.1.7 With an iron-sulfur protein as acceptor

1.1.9 With a copper protein as acceptor

1.1.98 With other, known, physiological acceptors

1.1.98.1 alcohol dehydrogenase (azurin)

1.1.98.2 glucose-6-phosphate dehydrogenase (coenzyme-F₄₂₀)

1.1.98.3 decaprenylphospho-β-D-ribofuranose 2-dehydrogenase

1.1.98.4 F₄₂₀H₂:quinone oxidoreductase

1.1.98.5 secondary-alcohol dehydrogenase (coenzyme-F₄₂₀)

1.1.98.6 ribonucleoside-triphosphate reductase (formate)

1.1.98.7 serine-type anaerobic sulfatase-maturating enzyme

1.1.99 With unknown physiological acceptors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.8.1 With NAD⁺ or NADP⁺ as acceptor

1.8.1.1 created 1961, deleted 1972

1.8.1.2 assimilatory sulfite reductase (NADPH)

1.8.1.3 hypotaurine dehydrogenase

1.8.1.4 dihydrolipoyl dehydrogenase

1.8.1.5 2-oxopropyl-CoM reductase (carboxylating)

1.8.1.6 cystine reductase

1.8.1.7 glutathione-disulfide reductase

1.8.1.8 protein-disulfide reductase

1.8.1.9 thioredoxin-disulfide reductase

1.8.1.10 CoA-glutathione reductase

1.8.1.11 asparagusate reductase

1.8.1.12 trypanothione-disulfide reductase

1.8.1.13 bis-γ-glutamylcystine reductase

1.8.1.14 CoA-disulfide reductase

1.8.1.15 mycothione reductase

1.8.1.16 glutathione amide reductase

1.8.1.17 dimethylsulfone reductase

1.8.1.18 NAD(P)H sulfur oxidoreductase (CoA-dependent)

1.8.1.19 sulfide dehydrogenase

1.8.1.20 4,4′-dithiodibutanoate disulfide reductase

1.8.1.21 dissimilatory dimethyldisulfide reductase

1.8.2 With a cytochrome as acceptor

1.8.3 With oxygen as acceptor

1.8.4 With a disulfide as acceptor

1.8.5 With a quinone or similar compound as acceptor

1.8.6 With a nitrogenous group as acceptor (deleted sub-subclass)

1.8.7 With an iron-sulfur protein as acceptor

1.8.98 With other, known, physiological acceptors

1.8.99 With unknown physiological acceptors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

3.1 Acting on ester bonds

3.2 Glycosylases

3.3 Acting on ether bonds

3.4 Acting on peptide bonds (peptidases)

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.6 Acting on acid anhydrides

3.7 Acting on carbon-carbon bonds

3.8 Acting on halide bonds

3.9 Acting on phosphorus-nitrogen bonds

3.10 Acting on sulfur-nitrogen bonds

3.11 Acting on carbon-phosphorus bonds

3.12 Acting on sulfur-sulfur bonds

3.13 Acting on carbon-sulfur bonds

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Release 2023.1 (January 2023)