EC Explorer

1.5.1.28

opine dehydrogenase

(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD⁺ + H₂O = L-2-aminopentanoic acid + pyruvate + NADH + H⁺

(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD⁺, L-aminopentanoate-forming)

(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD⁺ oxidoreductase (L-aminopentanoate-forming)

108281-02-3

In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate. In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor. They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine. The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.

created 1999