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EC 1.14.14.32 Details
EC number
1.14.14.32
Accepted name
17α-hydroxyprogesterone deacetylase
Reaction
(1) 17α-hydroxyprogesterone + [reduced NADPH—hemoprotein reductase] + O2 = androstenedione + acetate + [oxidized NADPH—hemoprotein reductase] + H2O;;(2) 17α-hydroxypregnenolone + [reduced NADPH—hemoprotein reductase] + O2 = 3β-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH—hemoprotein reductase] + H2O
Other name(s)
C-17/C-20 lyase, 17α-hydroxyprogesterone acetaldehyde-lyase, CYP17, CYP17A1 (gene name), 17α-hydroxyprogesterone 17,20-lyase
Systematic name
17α-hydroxyprogesterone,NADPH—hemoprotein reductase:oxygen oxidoreductase (17α-hydroxylating, acetate-releasing)
CAS registry number
62213-24-5
Comment
A microsomal cytochrome P-450 (heme-thiolate) protein that catalyses two independent reactions at the same active site - the 17-hydroxylation of pregnenolone and progesterone, which is part of glucocorticoid hormones biosynthesis (EC 1.14.14.19), and the conversion of the 17-hydroxylated products via a 17,20-lyase reaction to form androstenedione and 3β-hydroxyandrost-5-en-17-one, leading to sex hormone biosynthesis. The activity of this reaction is dependent on the allosteric interaction of the enzyme with cytochrome b5 without any transfer of electrons from the cytochrome [2,4]. The enzymes from different organisms differ in their substrate specificity. While the enzymes from pig, hamster, and rat accept both 17α-hydroxyprogesterone and 17α-hydroxypregnenolone, the enzymes from human, bovine, sheep, goat, and bison do not accept the former, and the enzyme from guinea pig does not accept the latter [1].
History
created 1976 as EC 4.1.2.30, transferred 2016 to EC 1.14.14.32
EC Tree
3.1.1.9 created 1961, deleted 1972
3.1.1.12 created 1961, deleted 1972
3.1.1.16 created 1961, deleted 1972
3.1.1.18 created 1961, deleted 1982
3.1.1.62 created 1989, deleted 1992
3.1.1.69 created 1992, deleted 2002