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EC 3.4.22.65 Details
EC number
3.4.22.65
Accepted name
peptidase 1 (mite)
Reaction
Broad endopeptidase specificity
Other name(s)
allergen Der f 1, allergen Der p 1, antigen Der p 1, antigen Eur m 1, antigen Pso o 1, major mite fecal allergen Der p 1, Der p 1, Der f 1, Eur m 1, endopeptidase 1 (mite)
Comment
This enzyme, derived from the house dust mite, is a major component of the allergic immune response [2]. The substrate specificity of this enzyme is not altogether clear. It cleaves the low-affinity IgE receptor CD23 at Glu298┼Ser299 and Ser155┼Ser156 [1]. It also cleaves the pulmonary structural proteins occludin and claudin at Leu┼Leu, Asp┼Leu and at Gly┼Thr bonds [1,2]. It can also cleave the α subunit of the interleukin-2 (IL-2) receptor (CD25) [4]. Using a positional scanning combinatorial library, it was found that the major substrate-specificity determinant is for Ala in the P2 position [3]. The enzyme shows only a slight preference for basic amino acids in the P1 and P3 positions and a preference for aliphatic amino acids such as Ile, Pro, Val, Leu and norleucine in the P4 position [3]. Belongs in peptidase family C1A.
History
created 2007
EC Tree
3.1.1.9 created 1961, deleted 1972
3.1.1.12 created 1961, deleted 1972
3.1.1.16 created 1961, deleted 1972
3.1.1.18 created 1961, deleted 1982
3.1.1.62 created 1989, deleted 1992
3.1.1.69 created 1992, deleted 2002