EC Explorer

1.5.99.12

cytokinin dehydrogenase

N⁶-prenyladenine + acceptor + H₂O = adenine + 3-methylbut-2-enal + reduced acceptor

N⁶-dimethylallyladenine:(acceptor) oxidoreductase, 6-N-dimethylallyladenine:acceptor oxidoreductase, OsCKX2, CKX, cytokinin oxidase/dehydrogenase, N⁶-dimethylallyladenine:acceptor oxidoreductase

N⁶-prenyladenine:acceptor oxidoreductase

55326-39-1

A flavoprotein (FAD). Catalyses the oxidation of cytokinins, a family of N⁶-substituted adenine derivatives that are plant hormones, where the substituent is a prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and copper(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].

created 2001