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7.5.2.1: ABC-type maltose transporter

This is an abbreviated version!
For detailed information about ABC-type maltose transporter, go to the full flat file.

Word Map on EC 7.5.2.1

Reaction

ATP
+
H2O
 +
maltose-[maltose-binding protein][side 1]
=
ADP
 +
phosphate
 +
maltose[side 2]
 +
[maltose-binding protein][side 1]

Synonyms

ATP phosphohydrolase (maltose-importing), Cg2463, EC 3.6.3.19, GlvC, Mal-EFGK2, Mal11, MalE, MalE-FGK2, MalF, MalFGK2, MalFGK2-E, MalG511, MalK, MalK2, malK3, MalP, MalT, MALT2, MALT4, maltose ABC transporter, maltose ABC transporter MALFGK2-E, maltose ABC-type transporter, maltose ATP binding cassette transporter, maltose ATP-binding cassette importer, maltose ATP-binding cassette transporter, maltose importer, maltose transporter, maltose transporter ATPase, maltose transporter MalFGK2, maltose-specific enzyme IICB, maltose-transporting ATPase, maltose/maltodextrin ABC transporter, More, MuSK, proton-maltose symporter

ECTree

     7 Translocases
         7.5 Catalysing the translocation of carbohydrates and their derivatives
             7.5.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.5.2.1 ABC-type maltose transporter

Crystallization

Crystallization on EC 7.5.2.1 - ABC-type maltose transporter

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
MalE domain, sitting drop vapor diffusion method, using 100 mM BisTris, pH 5.5, 0.2 M LiSO4, 25% (w/v) PEG 3350
crystal structure analysis of full transporter MalFGK2 and of the MalF subunit
-
crystal structure analysis, e.g. of the crystal structure of the complex in the presence of ATP and MalE, containing MalK mutant E159Q, is resolved at 2.8 A, revealing that MalFG are in a periplasmically open state
enzyme IIA in complex with the maltose transporter, vapor diffusion method, using 100 mM sodium cacodylate pH 5.6, 13% (w/v) PEG monomethylether 2000 and 100 mM NDSB-256
MalK crystal structure analysis in the semi-open, nucleotide-free, the open nucleotide-free, the ATP-bound, and the ADP-bound states
pre-translocation, outward- and inward-facing crystal structure analysis of enzyme complex with or without bound maltose binding protein and bound maltose and ATP, overview
-
purified native MalFGK2 and and selenium methionine-labeled MalFGK2, in 20 mM Tris, pH 8.0, 50 mM NaCl, and 0.03% n-undecyl-beta-D-maltopyranoside, addtion of 5 mM 2-mercaptoethanol and 5 mM DTT for the SeMet-enzyme. Mixing of 1 mg/ml protein and reservoir solution, containing 11.5% PEG 4000, 0.1M N-(2-acetamido)iminodiacetic acid, pH 6.5, 0.1 M NaCl, and 0.1 M Li2SO4, in a 2:1 ratio, at 20°C in sitting drops, crystals appear after 24 h and grow to full size within 1 week, X-ray diffraction structure determination and anaylsis at 2.8 A resolution