7.4.2.7: ABC-type alpha-factor-pheromone transporter
This is an abbreviated version!
For detailed information about ABC-type alpha-factor-pheromone transporter, go to the full flat file.
Reaction
Synonyms
ABC transporter, ABC transporter Ste6, adenosine triphosphate-binding cassette transporter, alpha-factor transporter Ste6, alpha-factor-transporting ATPase, ATP-binding cassette (ABC) transporter Ste6, EC 3.6.3.48, HST6 gene product, More, Ste6, STE6 gene product, Ste6p, yeast alpha-factor transporter
ECTree
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Engineering
Engineering on EC 7.4.2.7 - ABC-type alpha-factor-pheromone transporter
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A517P
mutant enzyme shows 0.01% of the mating efficiency of the wild-type enzyme
A517T
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
A532H
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
DELTA-Abox
-
concurrent loss of phosphorylation and ubiquitination in the A-box mutant
E439K
mutant enzyme shows 0.01% of the mating efficiency of the wild-type enzyme
E531K/V533I
mutant enzyme shows 0.75% of the mating efficiency of the wild-type enzyme
F630L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
F656L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
G320D
mutant enzyme shows 5.5% of the mating efficiency of the wild-type enzyme
G320V
mutant enzyme shows 6% of the mating efficiency of the wild-type enzyme
G38D
mutant enzyme is completly defective for mating, mutant enzyme does not accumulate in plasma membrane fraction
G38N
mutant enzyme shows 0.06-0.01% of the mating efficiency of the wild-type enzyme
G397N
mutant enzyme shows 1.7% of the mating efficiency of the wild-type enzyme
G509D
-
significant reduction in alpha-factor transport. The organism shows 56fold decrease in mating relative to wild-type control
I521N
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
P525L
mutant enzyme shows 7% of the mating efficiency of the wild-type enzyme at 25°C, 0.06% at 30°C and is defective for mating at 37°C
R518T
-
mates with wild-type efficiency at 30°C. The organism shows 11fold reduction in mating at 37°C
R833K/R941H
mutant enzyme shows 1.9% of the mating efficiency of the wild-type enzyme
S507I
-
less severe reduction in alpha-factor transport. The organism shows a mating defect at 30°C
S507N
S507N/G508S/G509R
mutant enzyme is completely defective for mating
S507R
-
significant reduction in alpha-factor transport. The organism shows a mating defect at 30°C
S623L
-
3fold increased stability compared to wild-type enzyme, phosphorylation is reduced
S632F/Q633STOP
mutant enzyme shows 1.8% of the mating efficiency of the wild-type enzyme
T613A
-
2fold increased stability compared to wild-type enzyme, phosphorylation is not affected
T613A/S623L
-
5fold increased stability compared to wild-type enzyme
Y648L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y657L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y661L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y713L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
DELTA-Abox
-
concurrent loss of phosphorylation and ubiquitination in the A-box mutant
-
S623L
-
3fold increased stability compared to wild-type enzyme, phosphorylation is reduced
-
T613A
-
2fold increased stability compared to wild-type enzyme, phosphorylation is not affected
-
T613A/S623L
-
5fold increased stability compared to wild-type enzyme
-
-
significant reduction in alpha-factor transport. The organism shows 90fold decrease in mating relative to the wild-type control
S507N
mutant enzyme shows 0.5-0.85% of the mating efficiency of the wild-type enzyme