7.3.2.4: ABC-type nitrate transporter
This is an abbreviated version!
For detailed information about ABC-type nitrate transporter, go to the full flat file.
Word Map on EC 7.3.2.4
Reaction
Synonyms
ABC-type nitrate/nitrite transporter, ABC-type nitrate/nitrite-bispecific transporter, ABC-type NRT, AtNRT2.1, ATNRT2.7, ATP-binding cassette-type nitrate/nitrite-bispecific transporter, BcNRT1, BnNrt1.1, BnNrt2.1, CHL1, CHL1 NO3- transporter, CynABD transporter, EC 3.6.3.26, high-affinity NO3- transporter, homologue of nitrate polytopic membrane transporter, More, narK gene product, NarK1, NarK2, NarU, NAX1, NIA1, Nia2, NIP/LATD, nitrate transporter, nitrate transporter 1/peptide transporter family 6.8, nitrate transporter 2.5, nitrate transporter CHL1, nitrate transporter chlorate resistant 1, nitrate-transporting ATPase, nitrate/nitrite ABC transporter, nitrate/nitrite transporter, NO3- transporter, NPF2.3, NPF2.4, NPF6.3, NPF6.8, NPF7.3, NRT1, NRT1.1, NRT1.1 NO3- transporter, NRT1.1b, NRT1.2, NRT1.3, NRT1.4, NRT1.5, NRT1.6, NRT1.7, NRT1.8, NRT1.9, NRT2, NRT2.1, NRT2.2, NRT2.4, NRT2.5, NRT2.7, NrtP, NrtP permease, periplasmic nitrate/nitrite binding subunit of NRT, pNRT2.1, type I H+/nitrate symporter, xylem nitrate loading transporter, Ynt1, YNT1 gene product
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7.3.2.4 ABC-type nitrate transporterAdvanced search results
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results (Engineering
Engineering on EC 7.3.2.4 - ABC-type nitrate transporter
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H356A
the mutation results in complete loss of nitrate binding ability
T101A
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inactivation of the high-affinity component of the transporter by preventing phosphorylation at Thr101
T101D
the variant shows increased nitrate uptake of approximately 2.8fold compared to the wild type enzyme
F265A
the mutant retains very weak binding to nitrate but not to nitrite
N173A
the mutant retains some binding to substrate, albeit drastically reduced in comparison to the wild type
R303A
the mutation completely abrogates binding to nitrate or nitrite
Y261A,
the mutation completely abrogates binding to nitrate or nitrite
D267G
the mutant shows reduced activity compared to the wild type enzyme
F114S
the mutant shows reduced activity compared to the wild type enzyme
F19S
the mutant shows reduced activity compared to the wild type enzyme
G140S
the mutant shows reduced activity compared to the wild type enzyme
G315S
the mutant shows reduced activity compared to the wild type enzyme
G69S
the mutant shows reduced activity compared to the wild type enzyme
P50S
the mutant shows reduced activity compared to the wild type enzyme
P84T
the mutant shows reduced activity compared to the wild type enzyme
G140S
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the mutant shows reduced activity compared to the wild type enzyme
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G69S
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the mutant shows reduced activity compared to the wild type enzyme
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K243R/K253R/K270R
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no ubiquitinylation, strongly reduced down-regulation in response to glutamine
additional information
additional information
construction of a knockout mutant, no effect on plant growth, no changes in nitrate uptake upon acid load or medium acidification
additional information
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mutation of the NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport, knockdown or knockout mutations of NRT1.5 reduces the amount of nitrate transported from the root to the shoot, Root and shoot nitrate contents of antisense NRT1.5 plants, overview. T-DNA-tagged mutants nrt1.5-1 and nrt1.5-2 are defective in long-distance transport of nitrate but not of sulfate or phosphate
additional information
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a line expressing the auxin-inducible DR5::GUS reporter gene is crossed with the chl1-5 knockout mutant for NRT1.1. Mutation of NRT1.1 does not affect expression of DR5::GUS in plants supplied with 1 mM NO3, but dramatically increases it in plants either grown in the absence of NO3- or supplied with a low external NO3- concentration. Absence of a functional NRT1.1 transporter prevents the decrease of DR5::GUS expression in response to removal or lowered supply of NO3-, leading to a high DR5 activity regardless of the presence of an N source, phenotype, overview
additional information
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isolation of three high nitrogen insensitive mutants, belonging to three genetic loci and related to single and recessive mutations, that display reduced downregulation of both NRT2.1 expression and high-affinity NO3- influx under repressive conditions. associated with an almost complete suppression of systemic repression of pNRT2.1 activity by high N status of the whole plant. The three hni mutants define a class of N signaling mutants specifically impaired in the systemic feedback repression of root NO3- uptake, molecular effects of the mutations and phenotypes, detailed overview. pNRT2.1::LUC is a good marker for NRT2.1 expression
additional information
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generation of several mutants devoid of NAR2.1, e.g. Atnar2.1-35S:NAR2.1-myc mutant, complementation by expression of wild-type NAR2.1, phenotypes, overview
additional information
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mutation of the NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport, knockdown or knockout mutations of NRT1.5 reduces the amount of nitrate transported from the root to the shoot, Root and shoot nitrate contents of antisense NRT1.5 plants, overview. T-DNA-tagged mutants nrt1.5-1 and nrt1.5-2 are defective in long-distance transport of nitrate but not of sulfate or phosphate
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additional information
nitrate uptake is restored in mutant chl1-5 plants by expression of the wild-type enzyme using the 35S::BcNRT1 construct
additional information
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construction of deletion mutants, NarK1 mutant grows almost normally, while NarK2 mutant shows strongly reduced generation times under nitrate-dependent anaerobic growth conditions, almost no growth of the NarK1/NarK2 double mutant, impaired growth can be restored by expression of the narK gene from Escherichia coli K-12
additional information
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the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview
additional information
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the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview
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