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7.3.2.4: ABC-type nitrate transporter

This is an abbreviated version!
For detailed information about ABC-type nitrate transporter, go to the full flat file.

Word Map on EC 7.3.2.4

Reaction

ATP
+
H2O
 +
nitrate-[nitrate-binding protein][side 1]
=
ADP
 +
phosphate
 +
nitrate[side 2]
 +
[nitrate-binding protein][side 1]

Synonyms

ABC-type nitrate/nitrite transporter, ABC-type nitrate/nitrite-bispecific transporter, ABC-type NRT, AtNRT2.1, ATNRT2.7, ATP-binding cassette-type nitrate/nitrite-bispecific transporter, BcNRT1, BnNrt1.1, BnNrt2.1, CHL1, CHL1 NO3- transporter, CynABD transporter, EC 3.6.3.26, high-affinity NO3- transporter, homologue of nitrate polytopic membrane transporter, More, narK gene product, NarK1, NarK2, NarU, NAX1, NIA1, Nia2, NIP/LATD, nitrate transporter, nitrate transporter 1/peptide transporter family 6.8, nitrate transporter 2.5, nitrate transporter CHL1, nitrate transporter chlorate resistant 1, nitrate-transporting ATPase, nitrate/nitrite ABC transporter, nitrate/nitrite transporter, NO3- transporter, NPF2.3, NPF2.4, NPF6.3, NPF6.8, NPF7.3, NRT1, NRT1.1, NRT1.1 NO3- transporter, NRT1.1b, NRT1.2, NRT1.3, NRT1.4, NRT1.5, NRT1.6, NRT1.7, NRT1.8, NRT1.9, NRT2, NRT2.1, NRT2.2, NRT2.4, NRT2.5, NRT2.7, NrtP, NrtP permease, periplasmic nitrate/nitrite binding subunit of NRT, pNRT2.1, type I H+/nitrate symporter, xylem nitrate loading transporter, Ynt1, YNT1 gene product

ECTree

     7 Translocases
         7.3 Catalysing the translocation of inorganic anions and their chelates
             7.3.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.3.2.4 ABC-type nitrate transporter

Cloned

Cloned on EC 7.3.2.4 - ABC-type nitrate transporter

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a fusion protein between a 2-kb fragment of the ATNRT2.7 promoter and either the beta-glucuronidase or green fluorescent protein reporter gene stably introduced into the Arabidopsis thaliana ecotype Wassilewskija
-
ansient expression of the NRT1.8:enhanced green fluorescent protein fusion in onion epidermal cells and Arabidopsis protoplasts
-
expressed as GFP-fusion protein using Agrobacterium tumefaciens, correct localization
-
expressed as GUS-fusion protein in Arabidopsis thaliana
expressed in cells the nrtABCD deletion mutant NA3 of Synechococcus elongatus which restores the ability of the cell to grow on nitrate as sole nitrogen source
-
expressed in cultivar Oryza sativa japonica
-
expressed in Escherichia coli
expressed in Escherichia coli strain M15
-
expressed in High Five insect cells
expressed in Lactococcus lactis
-
expressed in Saccharomyces cerevisiae
expressed in Xenopus laevis oocytes
expressed in Xenopus spp. oocytes
-
expression of the pNRT2.1::LUC reporter gene construct in transgenic Arabidopsis thaliana plants, genotyping, overview
-
gene BcNRT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Brassica campestris mesophyll protoplasts from pEarleyGate101 and pEarleyGate104 vectors or the cauliflower mosaic virus 35S promoter using the BcNRT1 promoter for analysis of subcellular localization by YFP fluorescence labeling, expression analysis by quantitative RT-PCR
gene Medtr5g093170.1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of NRT1.3 in Xenopus laevis oocytes
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
heterologous expression of NRT2.4 in Xenopus laevis oocytes
-
mutants and GFP-fusion protein, GFP-fusion protein is active and correctly localized
-
narK gene expressed in NarK2 and NarK1/NarK2 deletion mutants of Pseudomonas aeruginosa PAO1, narK gene expression is capable of restoring the anaerobic growth of Pseudomonas aeruginosa PAO1, although at reduced rates compared with the wild type
-
the nitrite transporting system is encoded by the four genes nrtA, -B, -C, and -D, organized in the CynABD operon genes, physical map of the cynABDS genomic region
-
transient expression of GFP-NRT1.9 fusion protein in Arabidopsis thaliana mesophyll protoplasts using the CMV 35S promoter
-
truncated NrtA protein lacking the N-terminal 81 amino acids, expressed in Escherichia coli cells as a histidine-tagged soluble protein
-
Xenopus laevis oocytes are injected with nuclease-free water or ATNRT2.7 mRNA, ATNRT2.7 mRNA-injected oocytes take up significantly more nitrate than water-injected controls
-