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6.5.1.1: DNA ligase (ATP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP), go to the full flat file.

Word Map on EC 6.5.1.1

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.1 DNA ligase (ATP)

pH Range

pH Range on EC 6.5.1.1 - DNA ligase (ATP)

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 5
ligation activities, at 40°C under conditions of substrate saturation, are highest at pH 2.5-3.0, only slightly lower at pH 1.5-2.0 (about 80%) and are practically undetectable above pH 5.0
5 - 8.5
-
pH 5.0: about 65% of maximal activity, pH 8.5: about 45% of maximal activity, adenylyltransferase activity
6.5 - 8.5
6.5 - 9
approx. 20% of maximal activity at pH 6.5, approx. 90% of maximal activity at pH 9.0
6.9 - 8
Tequatrovirus T4
-
6.9: 46% of maximal activity, 8.0: 65% of maximal activity
6.9 - 8.3
Tequatrovirus T4
-
6.9: 40% of maximal activity, 8.3: 65% of maximal activity, joing of DNA
7 - 8.2
80% of maximal activity is observed between pH 7.0 and 8.2
7 - 8.5
-
approximately 70% of maximal activity at pH 7.0 and 8.5
7.2 - 8.4
-
7.2-7.7: maximal activity, 8.4: 50% of maximal activity
7.5 - 9.5
-
pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity