Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.5.1.1: DNA ligase (ATP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP), go to the full flat file.

Word Map on EC 6.5.1.1

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.1 DNA ligase (ATP)

Molecular Weight

Molecular Weight on EC 6.5.1.1 - DNA ligase (ATP)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
102000
-
sucrose density gradient centrifugation
104000
x * 104000, deduced from nucleotide sequence
105000
-
glycerol density gradient sedimentation
110000
-
x * 110000, SDS-PAGE
120000
-
x * 120000, SDS-PAGE
120000 - 130000
-
sucrose density gradient centrifugation, highly asymetric shape
125000
130000
175000
-
DNA ligase I, gel filtration, sucrose density gradient centrifugation
180000
190000
-
and a lower MW form of MW 95000, gel filtration
200000
-
gel filtration
240000
-
gel filtration
31000 - 32000
-
analytical ultracentrifugation
31540
-
gel filtration after preincubation with Mg2+ and diphosphate
31548
-
1 * 31548, deduced from nucleotide sequence
31873
-
1 * 31873, mass spectroscopy
41000
44000
-
x * 44000, DNA ligase V, SDS-PAGE
46000
-
1 * 46000 + 1 * 100000, DNA ligase III, SDS-PAGE. It cannot be concluded whether the 46000 MW peptide is an essential and integral part of DNA ligase III, or if it represents a separate protein that binds tentaciously to the 100000 MW DNA ligase component
52100
gel filtration
61000
-
enzyme-adenylate complex
62000
63000
64079
1 * 64079, deduced from nucleotide sequence
65000
-
1 * 65000, DNA ligase II, SDS-PAGE
67647
1 * 67647, deduced from nucleotide sequence
67800
-
x * 67800, calculation from sequence
68000
69000
1 * 69000, SDS-PAGE
69196
1 * 69196, the authors refer to the protein of 619 amino acids that is later reannotated as a protein of 602 amino acids (67747.6 Da), calculated from sequence
70000
gel filtration
75000
x * 75000, SDS-PAGE
79800
-
gel filtration
80000
82000
-
gel filtration
83000
-
1 * 83000, SDS-PAGE
84406
-
1 * 84406, calculation from nucleotide sequence
85000
86000
-
x * 86000, calculation from nucleotide sequence, SDS-PAGE
87000
-
x * 87000, SDS-PAGE
88000
-
1 * 88000, SDS-PAGE
90000
95000
98000
-
hydrodynamic measurements
additional information
-
DNA ligase I, and II have a markedly asymetric structure, gel filtration data lead to a gross overestimation of the MW