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6.5.1.1: DNA ligase (ATP)

This is an abbreviated version!
For detailed information about DNA ligase (ATP), go to the full flat file.

Word Map on EC 6.5.1.1

Reaction

(deoxyribonucleotide)n-3'-hydroxyl
+
5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m
=
(deoxyribonucleotide)n+m
 +
AMP

Synonyms

adenosine triphosphate-dependent ligase, ADL, APE1094, ApeLig, ATP-dependent DNA ligase, ATP-dependent DNA ligase 1, ATP-dependent DNA ligase I, ATP-dependent ligase, ATP-dependent ligase LigB, ATP-dependent ligase LigC, ATP-dependent ligase LigD, ATP-type DNA repair ligase, AtuLigD1, AtuLigD2, b-ADL, bacterial ATP-dependent DNA ligase, Cdc9, ChVLig, CVLig, Deoxyribonucleate ligase, Deoxyribonucleic acid joinase, Deoxyribonucleic acid ligase, Deoxyribonucleic acid repair enzyme, Deoxyribonucleic acid-joining enzyme, Deoxyribonucleic joinase, Deoxyribonucleic ligase, Deoxyribonucleic repair enzyme, Deoxyribonucleic-joining enzyme, DNA joinase, DNA ligase, DNA ligase 1, DNA ligase 4, DNA ligase D, DNA ligase I, DNA ligase II, DNA ligase III, DNA ligase IIIalpha, DNA ligase IV, DNA ligase IV homolog, DNA ligase IV-XRCC4 complex, DNA ligase IV/XRCC4 complex, DNA ligase IV/XRCC4/XLF complex, DNA ligase V, DNA ligase VI, DNA repair enzyme, DNA repair ligase D, DNA-joining enzyme, DNAligI, Dnl4, drB0100, Hbu DNA ligase, L3BRCT, LdMNPV DNA ligase, Lig, Lig E, Lig I, Lig III, LIG k alpha, Lig K protein, Lig(Tk), LIG1, Lig3, Lig3alpha, Lig4, LIG6, LigA, ligase 1, ligase D, ligase III, ligase III-alpha, LigB, LigC, LigC1, LigD, LigI, LigIII, LigTh1519, ligTK, MJ0171, MSH-1, Mt-Lig, Mth ligase, MtuLigB, MtuLigC, MtuLigD, NHEJ DNA repair ligase, PabDBD, PaeLigD, PBCV-1 DNA ligase, PF1635, PfLigI, Pfu DNA ligase, PfuLig, Polydeoxyribonucleotide synthase (ATP), Polydeoxyribonucleotide synthase [ATP], Polynucleotide ligase, Sealase, ssLig, SSO0189, T4 ATP ligase, T4 DNA ligase, T4 lig, Vaccinia ligase, Vib-Lig, X4L4, XRCC1/DNA ligase III, XRCC4-DNA ligase IV complex

ECTree

     6 Ligases
         6.5 Forming phosphoric-ester bonds
             6.5.1 Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
                6.5.1.1 DNA ligase (ATP)

Cloned

Cloned on EC 6.5.1.1 - DNA ligase (ATP)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amino acid sequence of human DNA ligase I and enzyme from Schizosaccharomyces pombe are virtually identical in the region of the active site, although the two enzymes show only 44% overall identity. The sequence of DNA ligase II appears to be quite different from that of DNA ligase I
-
an adenylate kinase-T4 DNA ligase fusion protein is expressed in Escherichia coli XL1-Blue cells
Tequatrovirus T4
-
ATP-dependent ligase LigB is produced in Escherichia coli as a His10-LigB fusion protein
-
ATP-dependent ligase LigC is produced in Escherichia coli as a His10-LigC fusion protein
-
ATP-dependent ligase LigD is produced in Escherichia coli as a His10-LigD fusion protein
-
cloned into two expression vectors. The resulting 6His-tagged proteins are overexpressed in Escherichia coli XL-10-Gold or BL-21 (DE3)
coexpression of DNA ligase IV-XRCC4 complex in Sf9 insect cells
-
DNA ligase I, III-alpha, III-beta, IV
-
DNA ligase II and IV. DNA ligase III and IV are encoded by distinct genes located on human chromosome 17q11.2-12 and 13q33-34
-
Escherichia coli-based coexpression system provides relatively high yields of the ligase IV/XRCC4 complex
expressed as His6-tagged protein in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli and in Sf9 insect cells
-
expressed in Escherichia coli as a His-tagged fusion protein
Paramecium bursaria chlorella virus
expressed in Escherichia coli as His-tagged enzyme
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 Star (DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
Tequatrovirus T4
-
expressed in Escherichia coli BL21(DE3)Star cells
expressed in Escherichia coli BL21-DE3-RIL cells
expressed in Escherichia coli BL21-Start cells
expressed in Escherichia coli BW23474 cells
-
expressed in Escherichia coli DLT1270/pRARE-2 cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli Rosetta-gami (DE3) cells
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
expressed in Escherichia coli strain DLT1270/pRARE
expressed in Escherichia coli strain ORIGAMI(DE3)pLysS
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli, polyhistidine-tagged enzyme
-
expression in Saccharomyces cerevisiae
-
expression in Sf9 insect cells
-
expression of domain 1, i.e. residues 1-240 and domain 2, i.e. residues 241-359 in Escherichia coli
-
expression of hemagglutinin-tagged DNA ligase in Crithidia fasciculata
expression of His-tagged DNA ligase I in Escherichia coli strain BL21(DE3)
expression of intein-DNA ligase I fusion protein in Escherichia coli
-
expression of wild-type and mutant Xrcc4/DNA ligase IV complexes in insect cells
-
gene sequence encoding FaLig is cloned into a bacterial expression vector harbouring an upstream His-tag to aid purification and expressed in Escherichia coli
-
Hbu DNA ligase gene is expressed under control of the T7lac promoter of pTARG in Escherichia coli BL21-Codon-Plus(DE3)-RIL
-
in vitro transcription and translation
in vitro transcription/translation, expression in Escherichia coli
overexpression in Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus
-
overexpression of wild-type and mutant enzymes in Escherichia coli
-
separate polymerase, nuclease and ligase domains of Mt-Lig are cloned individually, over-expressed in Escherichia coli B834
-
the catalytic domain of human LIG1 (residues 232-919) is expressed in Escherichia coli
-