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ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
ATP + L-Asp + L-Gln
AMP + diphosphate + L-Asn + L-Glu
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light, carbon and nitrogen availability control asparagine synthesis in sunflower by regulating three aspargine synthetase coding genes. HAS2 expression requires light and is positively affected by sucrose. HAS1 and HAS1.1 expression is dependent on nitrogen availability, while HAS2 transcripts are still found in N-starved plants. High ammonium level induces all three asparagine synthetase genes and partially reverts sucrose repression of HAS1 and HAS1.1
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
L-glutamine
L-glutamate + NH3
L-glutamine + H2O
L-glutamate + NH3
additional information
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ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
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the basic region leucine zipper protein ATF5, a transcriptional activator, stimulates asparagine promoter/reporter gene transcription via the nutrient-sensing response unit
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ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
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TaASN1 is dramatically induced by salinity, osmotic stress and exogenous abscisic acid
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ATP + L-Asp + L-Gln
AMP + diphosphate + Asn + Glu
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TaASN2 transcripts are very low in all detected tissues and conditions and are only slightly induced by abscisic acid in roots
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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glutamine is the in vivo nitrogen source
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine
AMP + diphosphate + L-asparagine + L-glutamate
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the enzyme might play a functional role in nitrogen translocation from root to aerial organs in Phaseolus vulgaris
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + L-glutamine + H2O
AMP + diphosphate + L-asparagine + L-glutamate
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
also reaction of EC 6.3.1.1
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
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L-glutamine
L-glutamate + NH3
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L-glutamine
L-glutamate + NH3
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L-glutamine
L-glutamate + NH3
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L-glutamine
L-glutamate + NH3
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L-glutamine
L-glutamate + NH3
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L-glutamine + H2O
L-glutamate + NH3
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L-glutamine + H2O
L-glutamate + NH3
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L-glutamine + H2O
L-glutamate + NH3
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L-glutamine + H2O
L-glutamate + NH3
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additional information
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physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
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additional information
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physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis
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additional information
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comprehensive mechanism has been proposed through which either Gln or NH3 can provide nitrogen for Asn production from Asp
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additional information
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major biosynthetic pathway for asparagine, AS gene expression is down-regulated by light
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additional information
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primary enzyme responsible for asparagine synthesis
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additional information
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Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
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additional information
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Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
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additional information
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Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
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additional information
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Helianthus annuus contains three asparagine synthetase genes: HAS1, HAS1.1 and HAS2. Most of the asparagine newly synthesized for germination and cotyledon expansion is due to HAS2 activity, with little contribution of the other asparagine synthetase genes. All three genes work together to synthesize asparagine for leaf senescence
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additional information
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upregulation of asparagine synthetase fails to avert cell cycle arrest induced by L-asparaginase in TEL/AML1-positive leukaemic cells
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additional information
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under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
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additional information
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under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
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additional information
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under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant
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additional information
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under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
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additional information
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under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
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additional information
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under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves
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additional information
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Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting
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additional information
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the primary site of Asn synthesis is the root and subsequently the leaves receive Asn as the principal N-source for amino acid and protein synthesis
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additional information
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enzyme is involved in ammonia assimilation
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additional information
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primary enzyme responsible for asparagine synthesis
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additional information
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Asn, the end product of the mass action of symbiotic NH4+ synthesis, is the principal N-transport-compound of many temperate legumes
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additional information
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glutamine or glutamine-derived metabolites regulate AS expression in roots
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additional information
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nitrogen metabolism, asparagine synthesis
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additional information
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role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
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additional information
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role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation.
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additional information
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primary enzyme responsible for asparagine synthesis
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additional information
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importance of asparagine synthetase in cell proliferation
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additional information
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possible involvement of the enzyme in the control of metabolic fluxes of carbon and nitrogen through assimilatory pathways
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