6.3.5.4: asparagine synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about asparagine synthase (glutamine-hydrolysing), go to the full flat file.

Reaction

Synonyms

AS, AS-A, AS-B, AS1, ASN3, AsnA, ASNase, AsnB, ASNS, AsnS1, AsnS2, AsnS3, AsnS4, asparagine amidotransferase, Asparagine synthetase, Asparagine synthetase (glutamine hydrolyzing), Asparagine synthetase (glutamine), Asparagine synthetase (glutamine-hydrolysing), asparagine synthetase 1, asparagine synthetase 2, Asparagine synthetase A, Asparagine synthetase B, asparagine synthetase, glutamine-dependent, asparagine synthetase1, asparagine synthetase2, At5g10240, bacterial type asparagine synthetase A, CaAS1, glutamine-dependent amidotransferase, Glutamine-dependent asparagine synthetase, HvAS1 protein, HvAS2 protein, HvASN1, HvASN2, HvASN3, HvASN4, HvASN5, L-asparaginase, L-Asparagine synthetase, LDBPK_300470, LiAS-A, MLOC_37219, MLOC_44080, MLOC_63089, MLOC_72774, MLOC_75057, More, NAS2, Os03g0291500, Os06g0265000, OsAS1, OsAS2, PVAS1 protein, PVAS2, Ste10, Synthetase, Asn (glutamine), TaASN1, TaASN2, TaASN4, TaSN1, TaSN2, TS11 cell cycle control protein, type -II asparagine synthetase, type I asparagine synthetase, type II asparagine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

                6.3.5.4 asparagine synthase (glutamine-hydrolysing)

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Results	in table
8	Activating Compound
20517	AA Sequence
12	Application
1	CAS Registry Number
60	Cloned(Commentary)
14	Cofactor
3	Crystallization (Commentary)
5649	Disease/ Diagnostics
7	Expression
46	General Information
7	General Stability
16	IC50 Value
176	Inhibitors
28	kcat/KM [mM/s]
28	Ki Value [mM]
165	KM Value [mM]
18	Localization
45	Metals/Ions
31	Molecular Weight [Da]
71	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
260	Organism
6	Pathway
6	PDB ID
23	pH Optimum
4	pH Range
1	pH Stability
6	pI Value
2	Posttranslational Modification
55	Protein Variants
34	Purification (Commentary)
12	Reaction
1	Reaction Type
148	Reference
3	Renatured (Commentary)
249	Source Tissue
11	Specific Activity [micromol/min/mg]
5	Storage Stability
206	Substrates and Products (Substrate)
32	Subunits
157	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
6	Temperature Stability [°C]
87	Turnover Number [1/s]

KM Value

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KM Value on EC 6.3.5.4 - asparagine synthase (glutamine-hydrolysing)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.53 - 55.7

Asp

13 entries

0.38

aspartic acid

Homo sapiens

-

pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme

660816

0.013 - 1.47

ATP

38 entries

0.16 - 5.24

Gln

14 entries

11.5 - 17.1

hydroxylamine

2 entries

0.13 - 1.2

L-Asp

19 entries

0.1 - 6.21

L-aspartate

9 entries

1.3

L-aspartic acid

Homo sapiens

-

pH 8, reaction with NH3, C-terminally tagged recombinant enzyme

660816

0.04 - 9

L-Gln

19 entries

0.09 - 0.26

L-glutamic acid gamma-monohydroxamate

3 entries

0.09 - 10.3

L-glutamine

13 entries

0.75 - 9.9

NH3

7 entries

2.1 - 120

NH4+

9 entries

additional information

additional information

12 entries

-

0.53

Asp

Escherichia coli

-

NH4+-dependent activity, wild-type

1702

0.6

Asp

Bos taurus

-

-

1674

0.68

Asp

Escherichia coli

-

wild-type enzyme

1698

0.85

Asp

Escherichia coli

-

Asp, wild-type enzyme, Gln-dependent activity

1700, 1702

0.85

Asp

Escherichia coli

-

C436A mutant

1698

0.88

Asp

Escherichia coli

-

C386A mutant

1698

0.96

Asp

Escherichia coli

-

C99A mutant

1698

1.24

Asp

Glycine max

-

-

1672

1.3

Asp

Lupinus luteus

-

-

1711

1.68

Asp

Escherichia coli

-

C168A mutant

1698

1.7

Asp

Escherichia coli

-

C514A mutant

1698

1.91

Asp

Escherichia coli

-

C423A mutant

1698

55.7

Asp

Escherichia coli

-

C523A mutant

1698

0.013

ATP

Escherichia coli

-

mutant E348D, glutamine-dependent activity, pH 8.0, 37°C

714228

0.013

ATP

Escherichia coli

-

mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C

714228

0.03

ATP

Escherichia coli

-

mutant E348D, ammonia-dependent activity, pH 8.0, 37°C

714228

0.03

ATP

Escherichia coli

-

mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C

714228

0.076

ATP

Glycine max

-

-

1672

0.08

ATP

Homo sapiens

-

pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme

660816

0.097

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.097

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3

716573

0.1

ATP

Escherichia coli

-

wild-type, glutamine-dependent activity, pH 8.0, 37°C

714228

0.1

ATP

Escherichia coli

-

wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C

714228

0.1 - 1

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1

716573

0.106

ATP

Glycine max

-

pH 7.6, 22°C

716573

0.11

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.11

ATP

Homo sapiens

-

pH 8, reaction with NH3, C-terminally tagged recombinant enzyme

660816

0.11

ATP

Escherichia coli

-

wild-type, ammonia-dependent activity, pH 8.0, 37°C

714228

0.11

ATP

Escherichia coli

-

wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C

714228

0.125

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.125

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant nontagged isozyme soluble ZmAsn2

716573

0.128

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.128

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2

716573

0.128

ATP

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4

716573

0.14

ATP

Rattus norvegicus

-

-

1688

0.14

ATP

Lupinus luteus

-

MgATP2-

1711

0.15

ATP

Lupinus albus

-

-

1709

0.18

ATP

Escherichia coli

-

wild-type

1698

0.18

ATP

Escherichia coli

-

glutamine dependent asparagine synthetase activity

649262

0.2

ATP

Mus musculus

-

-

858, 1713

0.2

ATP

Escherichia coli

-

ATP, C386A mutant

1698

0.21

ATP

Escherichia coli

-

C99A mutant

1698

0.24

ATP

Klebsiella aerogenes

-

ATP

861

0.24

ATP

Escherichia coli

-

C523A mutant

1698

0.26

ATP

Escherichia coli

-

-

649482

0.29

ATP

Escherichia coli

-

C436A mutant and C514A mutant

1698

0.39

ATP

Escherichia coli

-

C168A mutant

1698

0.44

ATP

Escherichia coli

-

C423A mutant

1698

0.7

ATP

Bos taurus

-

-

1674

1.2

ATP

Leishmania donovani

E9BLE1

recombinant enzyme, pH 7.8, 37°C

745294

1.47

ATP

Leishmania infantum

A4I213

pH 7.6, 37°C, recombinant enzyme

746172

0.16

Gln

Lupinus luteus

-

-

1711

0.16

Gln

Glycine max

-

-

1672

0.16

Gln

Medicago sativa

-

-

1690

0.37

Gln

Escherichia coli

-

C523A mutant

1698

0.51

Gln

Escherichia coli

-

C386A mutant

1698

0.55

Gln

Klebsiella aerogenes

-

-

861

0.57

Gln

Saccharomyces cerevisiae

-

-

1708

0.66

Gln

Escherichia coli

-

wild-type enzyme

1700, 1702

0.74

Gln

Escherichia coli

-

C436 mutant

1698

0.77

Gln

Escherichia coli

-

C423A mutant

1698

0.83

Gln

Escherichia coli

-

C168A mutant

1698

0.92

Gln

Escherichia coli

-

C514 mutant

1698

1.4

Gln

Bos taurus

-

-

1674

5.24

Gln

Escherichia coli

-

mutant C99A

1697

11.5

hydroxylamine

Escherichia coli

-

wild-type enzyme

1697

17.1

hydroxylamine

Escherichia coli

-

mutant N74A

1697

0.13

L-Asp

Escherichia coli

-

mutant E348D, glutamine-dependent activity, pH 8.0, 37°C

714228

0.13

L-Asp

Escherichia coli

-

mutant E348D, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C

714228

0.23

L-Asp

Escherichia coli

-

mutant E348D, ammonia-dependent activity, pH 8.0, 37°C

714228

0.23

L-Asp

Escherichia coli

-

mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C

714228

0.31

L-Asp

Rattus norvegicus

-

-

1710

0.58

L-Asp

Rattus norvegicus

-

-

858

0.58

L-Asp

Escherichia coli

-

wild-type, glutamine-dependent activity, pH 8.0, 37°C

714228

0.58

L-Asp

Escherichia coli

-

wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C

714228

0.76

L-Asp

Rattus norvegicus

-

-

1688

0.8

L-Asp

Lupinus albus

-

-

1709

0.85

L-Asp

Glycine max

-

pH 7.6, 22°C

716573

0.9

L-Asp

Mus musculus

-

-

858, 1713

0.9

L-Asp

Streptomyces sp.

Q8KN11

pH 7.4, 37°C

692123

0.91

L-Asp

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.93

L-Asp

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.98

L-Asp

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

1.2

L-Asp

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

1.2

L-Asp

Escherichia coli

-

wild-type, ammonia-dependent activity, pH 8.0, 37°C

714228

1.2

L-Asp

Escherichia coli

-

wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C

714228

0.1 - 2

L-aspartate

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3

716573

0.6

L-aspartate

Leishmania donovani

E9BLE1

recombinant enzyme, pH 7.8, 37°C

745294

0.68

L-aspartate

Escherichia coli

-

glutamine dependent asparagine synthetase activity

649262

0.85

L-aspartate

Escherichia coli

-

-

649482

0.9 - 1

L-aspartate

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant nontagged soluble isozyme ZmAsn2

716573

0.93

L-aspartate

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4

716573

0.98

L-aspartate

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1

716573

0.98

L-aspartate

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2

716573

6.21

L-aspartate

Leishmania infantum

A4I213

pH 7.6, 37°C, recombinant enzyme

746172

0.04

L-Gln

Lupinus albus

-

-

1709

0.04

L-Gln

Escherichia coli

-

mutant N74A

1697

0.11

L-Gln

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.233

L-Gln

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.254

L-Gln

Glycine max

-

pH 7.6, 22°C

716573

0.423

L-Gln

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.543

L-Gln

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

0.69

L-Gln

Escherichia coli

-

wild-type enzyme

1697, 1698

1

L-Gln

Mus musculus

-

-

858, 1713

1.1

L-Gln

Rattus norvegicus

-

-

858, 1715

1.1

L-Gln

Escherichia coli

-

mutant E348D, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present

714228

1.7

L-Gln

Escherichia coli

-

wild-type, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present

714228

2.7

L-Gln

Escherichia coli

-

mutant E348D, glutaminase activity, pH 8.0, 37°C, ATP absent

714228

3.5

L-Gln

Escherichia coli

-

mutant E348A, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present

714228

3.9

L-Gln

Escherichia coli

-

mutant E348Q, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present

714228

4.3

L-Gln

Rattus norvegicus

-

-

1688

5

L-Gln

Escherichia coli

-

wild-type, glutaminase activity, pH 8.0, 37°C, ATP absent

714228

5.8

L-Gln

Escherichia coli

-

mutant E348A, glutaminase activity, pH 8.0, 37°C, ATP absent

714228

9

L-Gln

Escherichia coli

-

mutant E348Q, glutaminase activity, pH 8.0, 37°C, ATP absent

714228

0.09

L-glutamic acid gamma-monohydroxamate

Escherichia coli

-

mutant N74A

1697

0.26

L-glutamic acid gamma-monohydroxamate

Escherichia coli

-

wild-type enzyme

1697

0.26

L-glutamic acid gamma-monohydroxamate

Escherichia coli

-

ATP, wild-type enzyme, Gln-dependent activity

1700

0.09

L-glutamine

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn2

716573

0.1 - 1

L-glutamine

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant nontagged soluble isozyme ZmAsn2

716573

0.233

L-glutamine

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn4

716573

0.423

L-glutamine

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn3

716573

0.543

L-glutamine

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, temperature not specified in the publication, recombinant His-tagged isozyme ZmAsn1

716573

0.69

L-glutamine

Escherichia coli

-

glutamine dependent asparagine synthetase activity

649262

0.69

L-glutamine

Escherichia coli

-

glutamine-dependent synthetase activity

649482

1.39

L-glutamine

Escherichia coli

-

glutaminase activity in the presence of ATP

649262

1.67

L-glutamine

Escherichia coli

-

glutaminase activity

649482

1.71

L-glutamine

Leishmania infantum

A4I213

pH 7.6, 37°C, recombinant enzyme

746172

1.9

L-glutamine

Escherichia coli

-

glutaminase activity in the absence of ATP

649262

1.9

L-glutamine

Homo sapiens

-

pH 8, C-terminally tagged recombinant enzyme

660816

10.3

L-glutamine

Leishmania donovani

E9BLE1

recombinant enzyme, pH 7.8, 37°C

745294

0.75

NH3

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

1.12

NH3

Leishmania infantum

A4I213

pH 7.6, 37°C, recombinant enzyme

746172

1.7

NH3

Homo sapiens

-

pH 8, C-terminally tagged recombinant enzyme

660816

5.95

NH3

Leishmania donovani

E9BLE1

recombinant enzyme, pH 7.8, 37°C

745294

8.4

NH3

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

9

NH3

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

9.9

NH3

Zea mays

B4FFJ0, B5U8J7, B5U8J8, B5U8J9

pH 7.6, 22°C

716573

2.1

NH4+

Lupinus albus

-

-

1709

2.7

NH4+

Medicago sativa

-

-

1690

9

NH4+

Mus musculus

-

-

858, 1713

10

NH4+

Rattus norvegicus

-

-

1688

13

NH4+

Escherichia coli

-

mutant N74A

1697

15.7

NH4+

Escherichia coli

-

wild-type enzyme

1697

17

NH4+

Escherichia coli

-

wild-type enzyme

1702

20

NH4+

Saccharomyces cerevisiae

-

-

1708

120

NH4+

Rattus norvegicus

-

-

858, 1715

additional information

additional information

Rattus norvegicus

-

-

1673

-

additional information

additional information

Klebsiella aerogenes

-

biphasic kinetic, linear portion near 0.5

861

-

additional information

additional information

Escherichia coli

-

Km-values of a number of site-specific mutant enzymes

1701

-

additional information

additional information

Zea mays

-

regulation: coregulation by light of the activities of three crucial enzymes of NH4+ assimilation and transport

1704

-

additional information

additional information

Escherichia coli

-

Km-values of mutant enzymes R30A, R30K, N74A, N74Q, and N79A

1700

-

additional information

additional information

Leishmania infantum

A4I213

steady-state kinetics

746172

-

additional information

additional information

Leishmania infantum

-

steady-state kinetics

746172

-

additional information

additional information

Escherichia coli

-

kinetic mechanism, kinetic model

649482

-

additional information

additional information

Zea mays

B4FFJ0

the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics

716573

-

additional information

additional information

Zea mays

B5U8J7

the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics

716573

-

additional information

additional information

Zea mays

B5U8J8

the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics

716573

-

additional information

additional information

Zea mays

B5U8J9

the AsnS isozymes are kinetically distinct with substantial differences in Km (Gln) and Vmax values, overview. None of the enzymes has cooperative enzyme kinetics

716573

-