Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.5.4: asparagine synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about asparagine synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.4

Reaction

ATP
+
L-aspartate
 +
L-glutamine
 +
H2O
=
AMP
 +
diphosphate
 +
L-asparagine
 +
L-glutamate

Synonyms

AS, AS-A, AS-B, AS1, ASN3, AsnA, ASNase, AsnB, ASNS, AsnS1, AsnS2, AsnS3, AsnS4, asparagine amidotransferase, Asparagine synthetase, Asparagine synthetase (glutamine hydrolyzing), Asparagine synthetase (glutamine), Asparagine synthetase (glutamine-hydrolysing), asparagine synthetase 1, asparagine synthetase 2, Asparagine synthetase A, Asparagine synthetase B, asparagine synthetase, glutamine-dependent, asparagine synthetase1, asparagine synthetase2, At5g10240, bacterial type asparagine synthetase A, CaAS1, glutamine-dependent amidotransferase, Glutamine-dependent asparagine synthetase, HvAS1 protein, HvAS2 protein, HvASN1, HvASN2, HvASN3, HvASN4, HvASN5, L-asparaginase, L-Asparagine synthetase, LDBPK_300470, LiAS-A, MLOC_37219, MLOC_44080, MLOC_63089, MLOC_72774, MLOC_75057, More, NAS2, Os03g0291500, Os06g0265000, OsAS1, OsAS2, PVAS1 protein, PVAS2, Ste10, Synthetase, Asn (glutamine), TaASN1, TaASN2, TaASN4, TaSN1, TaSN2, TS11 cell cycle control protein, type -II asparagine synthetase, type I asparagine synthetase, type II asparagine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.4 asparagine synthase (glutamine-hydrolysing)

Cloned

Cloned on EC 6.3.5.4 - asparagine synthase (glutamine-hydrolysing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid, expression in Escherichia coli
-
and overexpression
-
C-terminally tagged enzyme, baculovirus-based expression system, the recombinant enzyme is correctly processed, exhibits high activity and is stable on prolonged storage at -80°C
-
cDNA, from cell culture SB-P, sequencing
-
cloned into a 2 mü plasmid, pBS24.1GAS, suitable for replication in a Saccharomyces cerevisiae ciro strain AB116
-
cloned into a temperature-sensitive low copy plasmid, pOU71
-
cloning and specificity of the amino acid-dependent contol of its mRNA content
-
cloning of cDNA
-
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), enzyme expression analysis
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expression in Escherichia coli
expression of AS-GFP fusion protein in MOLT-4 cells
-
expression of several mutant enymes in Saccharomyces cerevisiae
-
from BmN4 cells, recombinant expression of the GFP-tagged or Venus fluorescent protein-tagged enzyme in BmN4 cells
gene ASN1, a single copy gene located on chromosome 5 of each genome, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain Rosetta DE3
gene ASN2, a single copy gene located on chromosome 3 of each genome, variety Chinese Spring lacks a TaASN2 gene in the B genome, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization, recombinant expression in Escherichia coli strain Rosetta DE3
gene ASN3, real-time quantitative RT-PCR isozyme expression analysis
gene ASN3, two copies of TaASN3 are found on chromosome 1 of each genome, termed TaASN3.1 and TaASN3.2, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization, recombinant expression in Escherichia coli strain Rosetta DE3
gene ASN4, a single copy gene located on chromosome 4 of each genome, DNA and amino acid sequence determination and analysis, sequence comparisons
gene ASNS, a single copy gene located on chromosome 7
gene asnS, expression of C-terminally His-tagged isozyme AsnS1 in Escherichia coli strains BL21(DE3) and Rosetta(DE3) mainly in the inclusion bodies
gene asnS2, expression of nontagged isozyme ZmAsnS2 in Escherichia coli, expression of C-terminally His-tagged isozyme AsnS2 in Escherichia coli strains BL21(DE3) and Rosetta(DE3) mainly in the inclusion bodies although small amounts of ZmAsnS2 are recovered in the soluble fraction
gene asnS3, expression of C-terminally His-tagged isozyme AsnS3 in Escherichia coli strains BL21(DE3) and Rosetta(DE3) mainly in the inclusion bodies
gene asnS4, expression of C-terminally His-tagged isozyme AsnS4 in Escherichia coli strains BL21(DE3) and Rosetta(DE3) mainly in the inclusion bodies
gene CaAS1, enzyme overexpression in transgenic Arabidopsis thaliana plants leads to enhanced resistance of the plants to Pseudomonas syringae pv. tomato DC3000 and Hyaloperonospora arabidopsidis, phenotypes, overview
-
gene HvASN1, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis
gene HvASN2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis
gene HvASN3, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis
gene HvASN4, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis
gene HvASN5, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis
gene LDBPK_300470, DNA and amino acid sequence determination and analysis, sequence comaparisons and phylogenetic analysis, cloning of the pSP-alpha-blast-alpha-ASNA and pSP72-alpha-neo-alpha-GFP-ASNA episome, overexpression of GFP-tagged ASNA in Leishmania donovani Bob strain MHOM/SD/62/1SCL2D, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21
gene NAS2, DNA and mino acid sequence determination and analysis, promoter determination, tissue expression analysis, overview
gene Os03g0291500, quantitative real-time PCR enzyme expression analysis
gene Os06g0265000, quantitative real-time PCR enzyme expression analysis
gene PpAS1, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis, genetic regulation of AS1 transcription, overview
gene PpAS2, DNA and amino acid sequence analysis, sequence comparisons, phylogenetic analysis
HvAS1, from leaves, located to the long arm of chromosome 5, sequencing
HvAS2, from leaves, located to the short arm of chromosome 3, sequencing
mutant enzyme in which the N-terminal Cys is replaced by Ala is expressed in Saccharomyces
-
overexpression in Escherichia col
-
PVAS1 gene, sequencing, expression in Escherichia coli
PVAS2 is cloned into the expression vector pGEXKG and overexpressed as a glutathione S-transferase fusion protein. PVAS2 is cloned into pUC18, expression in Escherichia coli asparagine-auxotroph ER4813
-
TaSN1 and TaSN2, expression in Escherichia coli
-
TaSN1, expression in Escherichia coli
-
three asparagine snythetase genes asnB, asnH and asnO, expression in Bacillus subtilis and in Escherichia coli ME6279, sequencing, genetic organization
-
two allelic genes ASN1 and ASN2
-
two cDNA clones LJAS1 and LJAS2, encoding different asparagine synthetases
-