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6.3.4.16: carbamoyl-phosphate synthase (ammonia)

This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (ammonia), go to the full flat file.

Word Map on EC 6.3.4.16

Reaction

2 ATP +

NH3
+
hydrogencarbonate
=
2 ADP
 +
phosphate
 +
Carbamoyl phosphate

Synonyms

arginine-specific CPS, CarB, carbamate kinase-like carbamoyl phosphate synthetase, carbamate kinase-like carbamoyl-phosphate synthetase, carbamoyl phosphate synthase-1, carbamoyl phosphate synthetase, carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase I, carbamoyl phosphate synthetase-1, carbamoyl-phosphate synthetase, carbamoyl-phosphate synthetase 1, Carbamoyl-phosphate synthetase I, Carbamoylphosphate synthase, Carbamoylphosphate synthase (ammonia), Carbamoylphosphate synthetase, Carbamoylphosphate synthetase (ammonia), Carbamoylphosphate synthetase I, carbamoylphosphate synthetase-I, Carbamyl phosphate synthase I, carbamyl phosphate synthetase I, carbamyl-phosphate synthetase I, Carbamylphosphate synthetase, carbamylphosphate synthetase 1, Carbamylphosphate synthetase I, carbmoylphosphate synthetase, Carbon-dioxide-ammonia ligase, Carbonate kinase (phosphorylating), CK-like CPS, CPS, CPS 1, CPS I, CPS I-like (ammonia- and N-acetyl-L-glutamate-dependent), CPS-1, CPS-I, CPS1, CPSase, CPSase I, CPSI, EC 2.7.2.5, mitochondrial carbamoylphosphate synthetase I, MS-s, Msm_0361, p165, urea-specific CPS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.16 carbamoyl-phosphate synthase (ammonia)

Engineering

Engineering on EC 6.3.4.16 - carbamoyl-phosphate synthase (ammonia)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A1378T
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
A438P
inactive
Al38P
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
C1327A
the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview
C1337A
the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview
G1376S
the mutation has no detectable effect on activity
L1381S
the mutation is clearly disease-causing (carbamoyl-phosphate synthetase deficiency), since it induces strong enzyme instability
L390R
the mutation is clearly disease-causing (carbamoyl-phosphate synthetase deficiency), since it induces strong enzyme instability
N355D
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
R1262X
-
a premature stop codon mutation naturally occuring in carbamoyl phosphate synthetase 1 deficiency, CPS1D
R803G
-
naturally occuring missense mutation involved in carbamoyl phosphate synthetase 1 deficiency, CPS1D
T1405N
-
significant change in CPSI enzymatic function
T1406D
naturally occurring single nucleotide polymorphism, phenotype, overview
T1443A
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
T344A
the mutation has no detectable effect on activity
T544M
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
W1410K
site-directed mutagenesis, the mutant binds N-acetyl-L-beta-phenylglutamate at the N-acetyl-L-glutamate binding site
Y389C
the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity
E261Q
-
no synthesis of carbonyl phosphate in presence of L-glutamine
K258L
-
synthesis of carbonyl phosphate in presence of L-glutamine
N1437D
-
site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme
N1440D
-
site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme
T1391V
-
site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme
T1394A
-
site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme
W1410K
-
site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme
D265A
-
no carbamoyl phosphate synthesis
D265E
-
about 10% of the specific acitivity of the wild-type enzyme
D265N
-
about 10% of the specific acitivity of the wild-type enzyme
D810A
-
no carbamoyl phosphate synthesis
D810E
-
no carbamoyl phosphate synthesis
D810N
-
no carbamoyl phosphate synthesis
H262N
-
no carbamoyl phosphate synthesis
H807N
-
about 30% of the specific acitivity of the wild-type enzyme
I316G
-
about 10% of the specific acitivity of the wild-type enzyme
I316H
-
no carbamoyl phosphate synthesis
I316S
-
about 30% of the specific acitivity of the wild-type enzyme
L229G
-
about 30% of the specific acitivity of the wild-type enzyme
S228A
-
about 30% of the specific acitivity of the wild-type enzyme
D265A
-
no carbamoyl phosphate synthesis
-
D265N
-
about 10% of the specific acitivity of the wild-type enzyme
-
H262N
-
no carbamoyl phosphate synthesis
-
L229G
-
about 30% of the specific acitivity of the wild-type enzyme
-
S228A
-
about 30% of the specific acitivity of the wild-type enzyme
-
additional information