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6.3.4.16: carbamoyl-phosphate synthase (ammonia)

This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (ammonia), go to the full flat file.

Word Map on EC 6.3.4.16

Reaction

2 ATP +

NH3
+
hydrogencarbonate
=
2 ADP
 +
phosphate
 +
Carbamoyl phosphate

Synonyms

arginine-specific CPS, CarB, carbamate kinase-like carbamoyl phosphate synthetase, carbamate kinase-like carbamoyl-phosphate synthetase, carbamoyl phosphate synthase-1, carbamoyl phosphate synthetase, carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase I, carbamoyl phosphate synthetase-1, carbamoyl-phosphate synthetase, carbamoyl-phosphate synthetase 1, Carbamoyl-phosphate synthetase I, Carbamoylphosphate synthase, Carbamoylphosphate synthase (ammonia), Carbamoylphosphate synthetase, Carbamoylphosphate synthetase (ammonia), Carbamoylphosphate synthetase I, carbamoylphosphate synthetase-I, Carbamyl phosphate synthase I, carbamyl phosphate synthetase I, carbamyl-phosphate synthetase I, Carbamylphosphate synthetase, carbamylphosphate synthetase 1, Carbamylphosphate synthetase I, carbmoylphosphate synthetase, Carbon-dioxide-ammonia ligase, Carbonate kinase (phosphorylating), CK-like CPS, CPS, CPS 1, CPS I, CPS I-like (ammonia- and N-acetyl-L-glutamate-dependent), CPS-1, CPS-I, CPS1, CPSase, CPSase I, CPSI, EC 2.7.2.5, mitochondrial carbamoylphosphate synthetase I, MS-s, Msm_0361, p165, urea-specific CPS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.16 carbamoyl-phosphate synthase (ammonia)

Cloned

Cloned on EC 6.3.4.16 - carbamoyl-phosphate synthase (ammonia)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of N-terminally His6-tagged wild-type and mutant CPSIs in Escherichia coli and expression in Spodopetra frugiperda SF9 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH10Bac cells
expression in Escherichia coli
-
expression in Escherichia coli and Schizosaccharomyces pombe
expression in Saccharomyces pombe
-
gene CPS1 is located on chromosome 2q35, genotyping and cloning of wild-type and mutant CPSIs in Cos7 and HeLa cells, which show increased activity with the wild-type gene, overview
-
gene CPS1, located on chromosome 2, genotyping, overview
gene CPSI, DNA and amino acid sequence determination and analysis, expression of the N-terminally His6-FLAG-tagged enzyme and mutant T1406D in Schizosaccharomyces pombe from pESP5, expression of mature protein of 1462 amino acid residues with the first 39 amino acids of the precursor protein replaced by a methionyl residue
gene CPSI, expression of the N-terminally His6-FLAG-tagged enzyme in Schizosaccharomyces pombe from pESP5