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6.3.3.2: 5-formyltetrahydrofolate cyclo-ligase

This is an abbreviated version!
For detailed information about 5-formyltetrahydrofolate cyclo-ligase, go to the full flat file.

Word Map on EC 6.3.3.2

Reaction

ATP
+
5-formyltetrahydrofolate
=
ADP
 +
phosphate
 +
5,10-methenyltetrahydrofolate

Synonyms

5,10-methenyl-tetrahydrofolate synthetase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-Methenyltetrahydropteroylglutamate synthetase, 5-CHO-THF cycloligase, 5-FCL, 5-Formyltetrahydrofolate cyclodehydrase, CH+-THF synthetase, Fau1p, Formyltetrahydrofolic cyclodehydrase, Methenyl THFS, Methenyl-THF synthetase, Methenyltetrahydrofolate synthetase, More, MTHFD1, MTHFS, N5-Formyltetrahydrofolic acid cyclodehydrase, Synthetase, methenyltetrahydrofolate, ygfA

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.3 Cyclo-ligases
                6.3.3.2 5-formyltetrahydrofolate cyclo-ligase

Purification

Purification on EC 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity chromatography and gel filtration
Q81LX0
recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis
recombinant His-tagged enzyme from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to over 95% homogeneity
-
recombinant truncation mutant comprising residues 6-202 from Escherichia coli strain BL21(DE3) by affinity chromatography and substrate competition elution
-
Talon metal affinity column chromatography
-
Talon metal affinity resin chromatography and Sephadex G-25 gel filtration
to near homogeneity, recombinant enzyme from Escherichia coli