6.3.2.9: UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase, go to the full flat file.

Word Map on EC 6.3.2.9

6.3.2.9

peptidoglycan

ligases

udp-murnac-pentapeptide

phosphinate

drug development

udp-n-acetylmuramyl-l-alanine

analysis

Reaction

UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate

Synonyms

D-Glutamate-adding enzyme, D-glutamic acid adding enzyme, D-Glutamic acid-adding enzyme, MurD, MurD cell wall enzyme, MurD ligase, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamate, UDP-Mur-Nac-L-Ala:L-Glu ligase, UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase, UDP-N-acetylmuramoyl-L-alanine:D-glutamateligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase, UDPMurNAc-L-alanine:D-glutamate ligase (ADP-forming), Uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase, Uridine diphospho-N-acetylmuramoylalanyl-D-glutamate synthetase

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.2 Acid—amino-acid ligases (peptide synthases)

6.3.2.9 UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

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Results	in table
39654	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
5	Cofactor
11	Crystallization (Commentary)
3	General Information
37	IC50 Value
90	Inhibitors
11	Ki Value [mM]
62	KM Value [mM]
7	Localization
22	Metals/Ions
5	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
171	Organism
7	Pathway
33	PDB ID
11	pH Optimum
2	Posttranslational Modification
22	Protein Variants
9	Purification (Commentary)
4	Reaction
2	Reaction Type
50	Reference
5	Source Tissue
5	Specific Activity [micromol/min/mg]
9	Storage Stability
49	Substrates and Products (Substrate)
3	Subunits
22	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
20	Turnover Number [1/s]

Purification

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Purification on EC 6.3.2.9 - UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

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3 entries

method that includes chromatography by a Talon resin

Staphylococcus aureus

P0A091

651400

method that includes DEAE-Trisacryl M and hydroxyapatite-Ultrogel chromatography. Large-scale purification using 2-mercaptoethanol as a reducing agent, results in 200 mg of purified enzyme from 54 l of culture, adducts with one or two molecules of 2-mercaptoethanol are formed. Replacement of 2-mercaptoethanol by dithiothreitol gave 162 mg of enzyme from 36 l of culture yielding no clusters with the reducing agent.

Escherichia coli

653712

recombinant His6-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and dialysis

Escherichia coli

P14900

746203

recombinant protein

Staphylococcus aureus

714367

recombinant recombinant GST-tagged MurD from Escherichia coli by glutathione affinity chromatography, His-tagged MurD from Mycobacterium smegmatis by nickel affinity chromatography

Mycobacterium tuberculosis

A5U4I2

690835