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6.3.2.9: UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase, go to the full flat file.

Word Map on EC 6.3.2.9

Reaction

ATP
+
UDP-N-acetyl-alpha-D-muramoyl-L-alanine
 +
D-glutamate
=
ADP
 +
phosphate
 +
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate

Synonyms

D-Glutamate-adding enzyme, D-glutamic acid adding enzyme, D-Glutamic acid-adding enzyme, MurD, MurD cell wall enzyme, MurD ligase, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamate, UDP-Mur-Nac-L-Ala:L-Glu ligase, UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase, UDP-N-acetylmuramoyl-L-alanine:D-glutamateligase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase, UDPMurNAc-L-alanine:D-glutamate ligase (ADP-forming), Uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase, Uridine diphospho-N-acetylmuramoylalanyl-D-glutamate synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.9 UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

Engineering

Engineering on EC 6.3.2.9 - UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D317A
-
reduced enzymatic activity with respect to the wild-type
D35A
-
reduced enzymatic activity with respect to the wild-type
E157A
-
reduced enzymatic activity with respect to the wild-type
E157K
-
reduced enzymatic activity with respect to the wild-type
H183A
-
reduced enzymatic activity with respect to the wild-type
H301A
-
reduced enzymatic activity with respect to the wild-type
K115A
-
reduced enzymatic activity with respect to the wild-type
K198A
-
reduced enzymatic activity with respect to the wild-type
K198F
-
reduced enzymatic activity with respect to the wild-type
N268A
-
reduced enzymatic activity with respect to the wild-type
N271A
-
reduced enzymatic activity with respect to the wild-type
R302A
-
reduced enzymatic activity with respect to the wild-type
R425A
-
reduced enzymatic activity with respect to the wild-type
Y194F
-
reduced enzymatic activity with respect to the wild-type
D35A
-
reduced enzymatic activity with respect to the wild-type
-
E157A
-
reduced enzymatic activity with respect to the wild-type
-
E157K
-
reduced enzymatic activity with respect to the wild-type
-
K115A
-
reduced enzymatic activity with respect to the wild-type
-
Y194F
-
reduced enzymatic activity with respect to the wild-type
-
TOF-123
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, glutamate-adding activity of mutant enzyme TOF-9 is about 17% of wild-type at 30 C and less at 43 C, no glutamate-adding activity can be detected in TOF-123 mutant enzyme, activity of mutant enzyme TOF-1 is marginally reduced to about 85% of wild type
TOF-9
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, glutamate-adding activity of mutant enzyme TOF-9 is about 17% of wild-type at 30 C and less at 43 C, no glutamate-adding activity can be detected in TOF-123 mutant enzyme, activity of mutant enzyme TOF-1 is marginally reduced to about 85% of wild type
TOF-95
-
mutant enzyme TOF-95 is almost indistinguishable from wild type, glutamate-adding activity of mutant enzyme TOF-9 is about 17% of wild-type at 30 C and less at 43 C, no glutamate-adding activity can be detected in TOF-123 mutant enzyme, activity of mutant enzyme TOF-1 is marginally reduced to about 85% of wild type