6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Reaction

Synonyms

Asuc_1947, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -glutamate-cysteine ligase-glutathione synthetase, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GCL, GCSGS, ghF, glutamate cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, Glutathione synthase, Glutathione synthetase, Glutathione synthetase (tripeptide), GS, GSH synthase, GSH synthetase, GSH-S, GSH2, gshAB, GSHase, gshB, GshF, GshFAp, GshFAs, GSHII, GSHS, GSHS1, GSS, hGS, L-glutathione synthetase, More, Phytochelatin synthetase, StGCL-GS, Synthetase, glutathione, TAGS1, TaGS2, TbGS, ZmGS

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.3 glutathione synthase

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Results	in table
15	Activating Compound
21524	AA Sequence
8	Application
1	CAS Registry Number
64	Cloned(Commentary)
43	Cofactor
13	Crystallization (Commentary)
527	Disease/ Diagnostics
2	Expression
28	General Information
6	General Stability
1	IC50 Value
82	Inhibitors
9	kcat/KM [mM/s]
19	Ki Value [mM]
216	KM Value [mM]
19	Localization
62	Metals/Ions
40	Molecular Weight [Da]
62	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
1904	Organism
7	Pathway
29	PDB ID
35	pH Optimum
1	pH Range
2	pH Stability
4	pI Value
7	Posttranslational Modification
99	Protein Variants
32	Purification (Commentary)
9	Reaction
2	Reaction Type
141	Reference
74	Source Tissue
39	Specific Activity [micromol/min/mg]
10	Storage Stability
241	Substrates and Products (Substrate)
36	Subunits
139	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
1	Temperature Range [°C]
20	Temperature Stability [°C]
103	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.071 - 55

ATP

29 entries

0.011 - 55

gamma-Glu-L-Cys

22 entries

13.6 - 21.7

gamma-L-Glu-aminobutanoate

6 entries

13

gamma-L-Glu-L-alpha-aminobutyrate

Rattus norvegicus

-

recombinant enzyme, pH 8.2, 37°C

649553

0.003 - 6.5

gamma-L-Glu-L-Cys

7 entries

1.17 - 15.68

gamma-L-glutamyl-L-cysteine

5 entries

0.052 - 55

Gly

24 entries

1.17 - 15.68

glycine

6 entries

additional information

additional information

3 entries

-

0.071

ATP

Arabidopsis thaliana

P46416

mutant enzyme R132K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.079

ATP

Arabidopsis thaliana

P46416

mutant enzyme R454A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.15

ATP

Arabidopsis thaliana

P46416

mutant enzyme K456M, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.17

ATP

Arabidopsis thaliana

P46416

mutant enzyme E371Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.45

ATP

Arabidopsis thaliana

P46416

mutant enzyme R274K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.61

ATP

Arabidopsis thaliana

P46416

mutant enzyme N376A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.72

ATP

Arabidopsis thaliana

P46416

mutant enzyme Q226A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.87

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 10°C, recombinant enzyme, ATPase activity

728286

1.46

ATP

Arabidopsis thaliana

P46416

mutant enzyme E220A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.593

ATP

Arabidopsis thaliana

P46416

mutant enzyme Q226N, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.85

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 15°C, recombinant enzyme, ATPase activity

728286

2.183

ATP

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

2.42

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 20°C, recombinant enzyme, ATPase activity

728286

2.94

ATP

Arabidopsis thaliana

P46416

mutant enzyme Q226N, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

2.94

ATP

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3 - 6

ATP

Arabidopsis thaliana

P46416

mutant enzyme E220Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.183

ATP

Arabidopsis thaliana

P46416

mutant enzyme E220Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.3

ATP

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

4.72

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 25°C, recombinant enzyme, ATPase activity

728286

4.99

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 28°C, recombinant enzyme, ATPase activity

728286

5.083

ATP

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

5.32

ATP

Pseudoalteromonas haloplanktis

Q3IFA2

pH 7.8, 30°C, recombinant enzyme, ATPase activity

728286

5.4

ATP

Synechocystis sp.

P73493

pH 7.5, 25°C, wild-type enzyme

726912

5.7

ATP

Arabidopsis thaliana

P46416

mutant enzyme S153A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

12.1

ATP

Arabidopsis thaliana

P46416

25°C, pH 7.5

662278

12.1

ATP

Arabidopsis thaliana

P46416

wild type enzyme, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

13

ATP

Rattus norvegicus

-

recombinant enzyme, pH 8.2, 37°C

649553

25.5

ATP

Pasteurella multocida

-

pH 8.2, 25°C

662457

55

ATP

Pasteurella multocida

-

inTris-HCl buffer (200 mM, pH 8.2)

662457

0.011

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme K456M, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.059

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme R132K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.077

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme R454A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.158

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme E371Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.383

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme N376A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.842

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme R274K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.973

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme Q226A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.485

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme Q226N, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.51

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme E220A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.13

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.13

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.25

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme E220Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.3

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme S153A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.517

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.817

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

5.317

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme S153A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

6.08

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme Q226A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

6.08

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

mutant enzyme Q226N, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

12.2

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

25°C, pH 7.5

662278

12.2

gamma-Glu-L-Cys

Arabidopsis thaliana

P46416

wild type enzyme, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

26.9

gamma-Glu-L-Cys

Pasteurella multocida

-

pH 8.2, 25°C

662457

55

gamma-Glu-L-Cys

Pasteurella multocida

-

inTris-HCl buffer (200 mM, pH 8.2)

662457

13.6

gamma-L-Glu-aminobutanoate

Homo sapiens

-

mutant V45W, pH 8.2, 37°C

714064

16.4

gamma-L-Glu-aminobutanoate

Homo sapiens

-

mutant V44W, pH 8.2, 37°C

714064

17.4

gamma-L-Glu-aminobutanoate

Homo sapiens

-

mutant V45A, pH 8.2, 37°C

714064

18.9

gamma-L-Glu-aminobutanoate

Homo sapiens

-

mutant V44A, pH 8.2, 37°C

714064

19.5

gamma-L-Glu-aminobutanoate

Homo sapiens

-

wild-type, pH 8.2, 37°C

714064

21.7

gamma-L-Glu-aminobutanoate

Homo sapiens

-

mutant V44A/V45A, pH 8.2, 37°C

714064

0.003

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant E144K and mutant N146D, pH 8.2, 37°C

652539

0.007

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant N146A, pH 8.2, 37°C

652539

0.008

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant K364A, pH 8.2, 37°C

652539

0.015

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant K364E, pH 8.2, 37°C

652539

0.336

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant K305E, pH 8.2, 37°C

652539

0.423

gamma-L-Glu-L-Cys

Homo sapiens

P48637

mutant K305A, pH 8.2, 37°C

652539

6.5

gamma-L-Glu-L-Cys

Homo sapiens

P48637

wild-type enzyme, pH 8.2, 37°C

652539

1.17

gamma-L-glutamyl-L-cysteine

Homo sapiens

P48637

pH 8.2, 37°C, D458R

726858

1.6

gamma-L-glutamyl-L-cysteine

Homo sapiens

P48637

pH 8.2, 37°C, D458A

726858

2.31

gamma-L-glutamyl-L-cysteine

Homo sapiens

P48637

pH 8.2, 37°C, D458N

726858

4.62

gamma-L-glutamyl-L-cysteine

Synechocystis sp.

P73493

pH 7.5, 25°C, wild-type enzyme

726912

15.68

gamma-L-glutamyl-L-cysteine

Homo sapiens

P48637

pH 8.2, 37°C, wild-type enzyme

726858

0.052

Gly

Homo sapiens

-

mutant G370V, 37°C, pH not specified in the publication

714035

0.062

Gly

Arabidopsis thaliana

P46416

mutant enzyme R132K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.08

Gly

Arabidopsis thaliana

P46416

mutant enzyme R454A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.133

Gly

Homo sapiens

-

mutant G369V, 37°C, pH not specified in the publication

714035

0.157

Gly

Arabidopsis thaliana

P46416

mutant enzyme E371Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.22

Gly

Arabidopsis thaliana

P46416

mutant enzyme R274K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.29

Gly

Arabidopsis thaliana

P46416

mutant enzyme K456M, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

0.37

Gly

Arabidopsis thaliana

P46416

mutant enzyme Q226A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.03

Gly

Arabidopsis thaliana

P46416

mutant enzyme N376A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.08

Gly

Arabidopsis thaliana

P46416

mutant enzyme E220A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

1.33

Gly

Arabidopsis thaliana

P46416

mutant enzyme Q226N, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

2.36

Gly

Homo sapiens

-

mutant G371V, 37°C, pH not specified in the publication

714035

2.467

Gly

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

2.583

Gly

Arabidopsis thaliana

P46416

mutant enzyme E220Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

2.94

Gly

Arabidopsis thaliana

P46416

mutant enzyme R454K, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3 - 6

Gly

Arabidopsis thaliana

P46416

mutant enzyme E220Q, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

3.3

Gly

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

5.183

Gly

Arabidopsis thaliana

P46416

mutant enzyme S155A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

7.2

Gly

Arabidopsis thaliana

P46416

mutant enzyme S153A, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

12.6

Gly

Arabidopsis thaliana

P46416

25°C, pH 7.5

662278

12.6

Gly

Arabidopsis thaliana

P46416

wild type enzyme, at 25°C in 100 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 2.5 mM gamma-glutamylcysteine, 10 mM glycine, 2.5 mM disodium ATP, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 5 units of type III rabbit muscle pyruvate kinase, and 10 units of type II rabbit muscle lactate dehydrogenase

674814

18.8

Gly

Homo sapiens

-

wild-type, 37°C, pH not specified in the publication

714035

36.3

Gly

Pasteurella multocida

-

pH 8.2, 25°C

662457

55

Gly

Pasteurella multocida

-

inTris-HCl buffer (200 mM, pH 8.2)

662457

1.17

glycine

Homo sapiens

P48637

pH 8.2, 37°C, D458R

726858

1.6

glycine

Homo sapiens

P48637

pH 8.2, 37°C, D458A

726858

2.31

glycine

Homo sapiens

P48637

pH 8.2, 37°C, D458N

726858

4.22

glycine

Synechocystis sp.

P73493

pH 7.5, 25°C, wild-type enzyme

726912

13

glycine

Rattus norvegicus

-

recombinant enzyme, pH 8.2, 37°C

649553

15.68

glycine

Homo sapiens

P48637

pH 8.2, 37°C, wild-type enzyme

726858

additional information

additional information

Escherichia coli

-

turnover-numbers of wild-type and mutant enzymes

1209

-

additional information

additional information

Escherichia coli

-

loopless mutant with a 930fold decrease in turnover number

1219

-

additional information

additional information

Escherichia coli

-

turnover number is fatally reduced in the P227V/G240V mutant

1218

-