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6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Word Map on EC 6.3.2.3

Reaction

ATP
+
gamma-L-glutamyl-L-cysteine
 +
glycine
=
ADP
 +
phosphate
 +
glutathione

Synonyms

Asuc_1947, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -glutamate-cysteine ligase-glutathione synthetase, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GCL, GCSGS, ghF, glutamate cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, Glutathione synthase, Glutathione synthetase, Glutathione synthetase (tripeptide), GS, GSH synthase, GSH synthetase, GSH-S, GSH2, gshAB, GSHase, gshB, GshF, GshFAp, GshFAs, GSHII, GSHS, GSHS1, GSS, hGS, L-glutathione synthetase, More, Phytochelatin synthetase, StGCL-GS, Synthetase, glutathione, TAGS1, TaGS2, TbGS, ZmGS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.3 glutathione synthase

Temperature Stability

Temperature Stability on EC 6.3.2.3 - glutathione synthase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-78
-
expression of the enzyme is absent after cryopreservation using increasing concentrations of DMSO at -78°C
0 - 30
purified recombinant enzyme, pH 7.8, 10 min, completely stable
37
-
stable below
39.3
mutant D24A enzyme,unfolding or transition midpoint (Tm)
41.3
-
melting temperature, mutant V45W
42.5
mutant R221A enzyme, unfolding or transition midpoint (Tm)
42.8
-
melting temperature, mutant V44A/V45A
47.8
-
melting temperature, mutant V45A
49.7
mutant S42A enzyme, unfolding or transition midpoint (Tm)
50
-
pH 8.3, 40% loss of activity after 1 h in 0.15 M Tris-HCl buffer
50.5
purified recombinant enzyme, pH 7.8, 10 min, 50% inactivation
51.1
-
melting temperature, mutant V44A
57
-
melting temperature, mutant V44W
60.3
wild-type enzyme unfolding or transition midpoint (Tm)
60.4
-
melting temperature, wild-type