6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Word Map on EC 6.3.2.3

Reaction

ATP
+
gamma-L-glutamyl-L-cysteine
+
glycine
=
ADP
+
phosphate
+
glutathione

Synonyms

More, Glutathione synthetase, Synthetase, glutathione, GSH synthetase, Glutathione synthetase (tripeptide), GSS, GS, Glutathione synthase, GSHase, Phytochelatin synthetase, gamma-GCS, GCL, GSHS1, GSHS, glutamate cysteine ligase, gamma-GCS-GS, GshF, ZmGS, gamma -glutamate-cysteine ligase-glutathione synthetase, GSH-S, TAGS1, TaGS2, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GSH2, GSH synthase, hGS, StGCL-GS, TbGS, gshB, bifunctional L-glutathione synthetase, Asuc_1947, gshAB, GshFAp, glutathione biosynthesis bifunctional protein GshAB, GshFAs, bifunctional glutathione synthetase, GCSGS, bifunctional GSH synthetase, ghF, GSHII, L-glutathione synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.3 glutathione synthase

Storage Stability

Storage Stability on EC 6.3.2.3 - glutathione synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, stable for at least 1 year
-
-20°C, 20% loss of activity after 2 months
-
-20°C, purified enzyme, 30% glycerol, at least 2 years stable
-
-20°C, stable for weeks
-
-80°C, 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, can be stored for up to 6 months without significant loss of activity
-
-80°C, in 50 mM Tris-HCl (pH 7.4) containing100 mM NaCl, up to 6 months, without significant loss of activity
-
4°C or frozen, in presence of glutathione and 5 mM DTT, stable for 1 month
-
4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol
-
4°C, when stored in 20 mM Tris-Cl and 1 mM EDTA, pH 8.6, in sterile cryogenic tubes at 4°C, the recombinant hGS dimer interface mutant enzymes lose activity in the 30 hours after purification. Both R221A and D24A lose activity in a biphasic manner within a few hours: D24A loses 30% of activity in 4 hours, while the activity of R221A decreases by 20% in 7.5 hours
stable for more than 2 months
-