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6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Word Map on EC 6.3.2.3

Reaction

ATP
+
gamma-L-glutamyl-L-cysteine
 +
glycine
=
ADP
 +
phosphate
 +
glutathione

Synonyms

Asuc_1947, bifunctional glutathione synthetase, bifunctional GSH synthetase, bifunctional L-glutathione synthetase, gamma -glutamate-cysteine ligase-glutathione synthetase, gamma-GCS, gamma-GCS-GS, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GCL, GCSGS, ghF, glutamate cysteine ligase, glutathione biosynthesis bifunctional protein GshAB, Glutathione synthase, Glutathione synthetase, Glutathione synthetase (tripeptide), GS, GSH synthase, GSH synthetase, GSH-S, GSH2, gshAB, GSHase, gshB, GshF, GshFAp, GshFAs, GSHII, GSHS, GSHS1, GSS, hGS, L-glutathione synthetase, More, Phytochelatin synthetase, StGCL-GS, Synthetase, glutathione, TAGS1, TaGS2, TbGS, ZmGS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.3 glutathione synthase

Purification

Purification on EC 6.3.2.3 - glutathione synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from kidney to homogeneity, partially from liver
-
from Plasmodium berghei infected mouse erythrocytes
-
His6-gamma-GCS-GS, bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities
-
Ni2+ affinity chromatography
Ni2+ affinity chromatography and gel filtration
Ni2+-NTA resin chromatography
-
nickel affinity chromatography and gel filtration chromatography
-
partial from brain
-
Q-Sepharose FF HR 16/10 column chromatography, butyl-Sepharose FF HR 16/10 column chromatography, HiPrep 26/10 desalting column chromatography, Source Q anion-exchange resin chromatography, and Superdex G-200 (16/60) gel filtration
-
recombinant enzyme
recombinant enzyme expressed in Escherichia coli, to homogeneity
-
recombinant from Escherichia coli BL21(DE3), to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by nickel affinity chromatography, gel filtration, and Cibacron blue 3GA affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Schizosaccharomyces pombe mutant strain, to homogeneity
-
recombinant His-tagged wild-type and mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type enzyme from Escherichia coli, to homogeneity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography