6.3.2.3: glutathione synthase

This is an abbreviated version!
For detailed information about glutathione synthase, go to the full flat file.

Word Map on EC 6.3.2.3

Reaction

ATP
+
gamma-L-glutamyl-L-cysteine
+
glycine
=
ADP
+
phosphate
+
glutathione

Synonyms

More, Glutathione synthetase, Synthetase, glutathione, GSH synthetase, Glutathione synthetase (tripeptide), GSS, GS, Glutathione synthase, GSHase, Phytochelatin synthetase, gamma-GCS, GCL, GSHS1, GSHS, glutamate cysteine ligase, gamma-GCS-GS, GshF, ZmGS, gamma -glutamate-cysteine ligase-glutathione synthetase, GSH-S, TAGS1, TaGS2, gamma-glutamate-cysteine ligase/glutathione synthetase, gamma-glutamylcysteine synthetase-glutathione synthetase, GSH2, GSH synthase, hGS, StGCL-GS, TbGS, gshB, bifunctional L-glutathione synthetase, Asuc_1947, gshAB, GshFAp, glutathione biosynthesis bifunctional protein GshAB, GshFAs, bifunctional glutathione synthetase, GCSGS, bifunctional GSH synthetase, ghF, GSHII, L-glutathione synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.3 glutathione synthase

Cloned

Cloned on EC 6.3.2.3 - glutathione synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and amplification of a gene for glutathione synthetase
-
cloning and complementation of a gsh2 mutant in fission yeast
-
cloning and sequencing of the large subunit
Saccharomyces pombe
-
cloning of the 2.2 kb 5'-flanking region, DNA sequence determination and analysis, promotor determination, determination of positive and negative regulation regions, e.g. NF1 repressor for induction of enzyme expression by tert-butylhydroquinone, expression in H4IIE cells, ATCC CRL-1548, via transformation by recombinant adenovirus vector
AF333982
cloning of the enzyme promoter and molecular mechanisms of GS transcriptional regulation, overview. Nrf1 and Nrf2 overexpression induces the human GS promoter activity. Human GS promoter contains two regions with homology to the nuclear factor erythroid 2, NFE2, motif that are required for basal activity as mutation of these sites reduces the human GS promoter activity by 66%
-
cloning of the enzyme promoter and molecular mechanisms of GS transcriptional regulation. The rat GS promoter contains functional AP-1 sites, some of which act as enhancers. It also contains a functional NF1 site that acts as a repressor, and basal expression requires AP-1 and NFkappaB, overview
-
cloning of the large subunit
-
DNA and amino acid sequence determination
DNA sequence analysis, overexpression of gene gshs2 in an enzyme-deficient Schizosaccharomyces pombe strain, expression of wild-type enzyme, isolated enzyme subunit fragments and mutants as C-terminally His-tagged proteins, subcloning in Escherichia coli DH5alpha
-
Escherichia coli B enzyme overproduced in Escherichia coli JM109
-
Escherichia coli C600 cells transformed by a recombinant plasmid for the glutathione synthetase gene of Escherichia coli B
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL-21DELTA(DE3) cells
expressed in Escherichia coli strain C41(DE3)
expressed in Escherichia coli strain K12
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli rosetta
-
expression in Escherichia coli, transformation into tobacco plants
expression in Schizosaccharomyces pombe
-
expression in Schizosaccharomyces pombe, results in 1.4fold higher glutathione content and 1.9fold ncreased enzyme activity, regulation by the Atf1-Spc-1-Wis1 signal pathway
-
expression of N-terminally His-tagged wild-type enzyme in Escherichia coli BL21(DE3)
gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha
gene GSH1 or GCL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3)
gene gsh2, physical mapping and genotyping, expression analysis, overview
gene GSH2, screening and DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21
gene gshB, expression of His-tagged wild-type and mutants
gene gshB, expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)
gene gshB, located on chromosome I, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gshB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gshF, recombinant expression in Escherichia coli strain JM109 from plasmid pTrc99A
gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3) coexpressing Escherichia coli acetate kinase (gene ack)
gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3), coexpression with acetate kinase (gene ack) from Lactobacillus sanfranciscensis
gene gshF, recombinant expression in Saccharomyces cerevisiae strains BY4717 and BY-G from plasmid JMB125 leading to increased levels of glutathione
gene gshs1, construction of cDNA library, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain 830, phylogenetic analysis reveals that gshs1 and gshs2, encoding homoglutathione synthetase, are a result of gene duplication, genomic organization
gene GSS, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL1 Blue
GS gene, located at chromosome 16F1, mapping of an X-linked arsenite-tolerance component to subdivision 16, overview
-
mutants Cys122Ala, Cys195Ala, Cys222Ala and Cys289Ala show no critical loss of activity. Multiple replacement of Cys residues, however, decreases enzymatic activity to 45-26% of the activity of the wild-type enzyme
-
Nrf1 is required for basal expression of GS in the mouse
-
overexpression in an Escherichia coli expression system
overexpression in Brassica juncea plants via infection with Agrobacterium tumefaciens, under control of the 35S cauliflower mosaic virus promotor
-
sequence comparison, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
seven naturally occurring missense mutations (L188P, D219A, D219G, Y270C, Y270H, R283C and P314L) are expressed using a His-tagged, Escherichia coli-based expression system
the gene for glutathione synthetase is polymerized and cloned onto the vector plasmid pBR325
-
the gene for glutathione synthetase of Escherichia coli B is cloned onto vector plasmid pBR325. Escherichia coli C600 cells transformed by a recombinant plasmid pBR325
-
wild-type and mutant enzymes, expression in Escherichia coli
-