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6.3.2.13: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase, go to the full flat file.

Word Map on EC 6.3.2.13

Reaction

ATP
+
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
 +
meso-2,6-diaminoheptanedioate
=
ADP
 +
phosphate
 +
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate

Synonyms

amide ligase, Diaminopimelic-adding enzyme, mDAP ligase, Meso-diaminopimelate-adding enzyme, Mur ligase, MurE, MurE synthetase, MurEEr, peptidoglycan MurE ligase, Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyl-meso-2,6-diaminopimelate, UDP-MurNAc-tripeptide synthetase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelate synthetase, UDP-N-acetylmuramyl tripeptide synthetase, UDP-N-acetylmuramyl-tripeptide synthetase, UDPMurNAc-L-alanyl-D-glutamate:mDAP ligase (ADP-forming), Uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase, Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.13 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

Cloned

Cloned on EC 6.3.2.13 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expresision of His-tagged MurE in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli strains BL21(DE3)/pLysS and DH5alpha
-
expression in Escherichia coli
expression of His-tagged MurE and selenomethionine-MurE in Escherichia coli
gene murE or Rv2158c, located in the division/cell wall (dcw) cluster,, identification of a promoter driving the co-transcription of mur synthetases along with key cell division genes such as ftsQ and ftsW, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3)/pLysS and Pseudomonas putida KT2442, subcloning in Escherichia coli strain DH5alpha.. Coexpression of genes murC/D/E/F/nat and genes pknA, pknB, murI, dapF, ddlA, namH, Rv2160c, ftsW, ftsQ, ftsZ, sepF, wag31 in Mycobacterium smegmatis
gene murE, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha