6.3.2.12: dihydrofolate synthase

This is an abbreviated version!
For detailed information about dihydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.12

Reaction

ATP
+
7,8-dihydropteroate
+
L-glutamate
=
ADP
+
phosphate
+
7,8-dihydropteroylglutamate

Synonyms

7,8-Dihydrofolate synthetase, 7,8-Dihydropteroate:L-glutamate ligase (ADP), Dihydrofolate synthetase, FHFS, FHFS/FPGS, Folylpoly-(gamma-glutamate) synthetase-dihydrofolate synthase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, Synthetase, dihydrofolate, DHFR, H2-folate synthetase, dihydrofolate synthetase-folylpolyglutamate synthetase, DHFS, dihydropteroate:L-glutamate ligase (ADP-forming), dihydrofolate reductase, FolC, dihydrofolate synthase, PfDHFS-FPGS, PNEJI1_000945, Rv2447c, LH57_13380

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.12 dihydrofolate synthase

Reference

Reference on EC 6.3.2.12 - dihydrofolate synthase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bognar, A.L.; Shane, B.
Bacterial folylpoly(gamma-glutamate) synthase-dihydrofolate synthase
Methods Enzymol.
122
349-359
1986
Corynebacterium sp.
Manually annotated by BRENDA team
Bognar, A.L.; Osborne, C.; Shane, B.; Singer, S.C.; Ferone, R.
Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product
J. Biol. Chem.
260
5625-5630
1985
Escherichia coli
Manually annotated by BRENDA team
Pongsamart, S.; Ho, R.I.; Corman, L.; Foye, W.O.
Characterization and inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae
Mol. Cell. Biochem.
59
165-171
1984
Neisseria gonorrhoeae
Manually annotated by BRENDA team
Shane, B.
Properties of Corynebacterium species dihydrofolate synthetase-folylpolyglutamate synthetase
Chem. Biol. Pteridines, Proc. Int. Symp. Pteridines Folic Acid Deriv. Chem. Biol. Clin. Aspects
621-626
1983
Corynebacterium sp.
-
Manually annotated by BRENDA team
Shane, B.; Cichowicz, D.J.
Folylpoly-gamma-glutamate synthetases: properties and regulation
Adv. Exp. Med. Biol.
163
149-165
1983
Corynebacterium sp.
Manually annotated by BRENDA team
Ferone, R.; Warskow, A.
Co-purification of dihydrofolate synthetase and N-10formyltetrahydropteroyldiglutamate synthetase from E. coli
Adv. Exp. Med. Biol.
163
167-181
1983
Escherichia coli
Manually annotated by BRENDA team
Pongsamart, S.; Ho, R.I.; Corman, L.; Foye, W.O.
Inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae by dihydropteroate and dihydrofolate derivatives
Chem. Biol. Interact.
36
369-373
1981
Neisseria gonorrhoeae
Manually annotated by BRENDA team
Iwai, K.; Ikeda, M.; Kobashi, M.
Intracellular distribution, purification, and properties of dihydrofolate synthetase from pea seedlings
Methods Enzymol.
66
581-585
1980
Pisum sativum
Manually annotated by BRENDA team
Ho, R.I.
A simple radioassay for dihydrofolate synthetase activity in Escherichia coli and its application to an inhibition study of new pteroate analogs
Methods Enzymol.
66
576-581
1980
Escherichia coli
Manually annotated by BRENDA team
Roland, S.; Ferone, R.; Harvey, R.J.; Styles, V.L.; Morrison, R.W.
The characteristics and significance of sulfonamides as substrates for Escherichia coli dihydropteroate synthase
J. Biol. Chem.
254
10337-10345
1979
Escherichia coli, Escherichia coli B / ATCC 11303
Manually annotated by BRENDA team
Ikeda, M.; Iwai, K.
Some characteristics of the dihydrofolate synthetase from Serratia indica
J. Nutr. Sci. Vitaminol.
22
365-373
1976
Serratia indica
Manually annotated by BRENDA team
Ikeda, M.; Iwai, K.
Purification and properties of the dihydrofolate synthetase from Serratia indica
J. Nutr. Sci. Vitaminol.
22
235-248
1976
Serratia indica
Manually annotated by BRENDA team
Ho, R.I.; Corman, L.; Ho, J.; Nair, M.G.
A simple radioassay for dihydrofolate synthetase activity in Escherichia coli and its applicationto an inhibition study of new pteroate analogs
Anal. Biochem.
73
493-500
1976
Escherichia coli, Neisseria meningitidis
Manually annotated by BRENDA team
Webb, S.R.; Ferone, R.
Inhibition of dihydrofolate synthetase by folate, homofolate, pteroate and homopteroate and their reduced forms
Biochim. Biophys. Acta
422
419-426
1976
Escherichia coli, Escherichia coli B / ATCC 11303
Manually annotated by BRENDA team
Iwai, K.; Ikeda, M.
Purification and properties of the dihydrofolate synthetase from pea seedlings
J. Nutr. Sci. Vitaminol.
21
7-18
1975
Pisum sativum
Manually annotated by BRENDA team
Ikeda, M.; Iwai, K.
The intracellular localization and stability of the dihydrofolate synthetase in pea seedlings
J. Nutr. Sci. Vitaminol.
21
1-6
1975
Pisum sativum, Spinacia oleracea
Manually annotated by BRENDA team
Brown, G.M.
Dihydrofolate synthetase
Methods Enzymol.
18B
771-775
1971
Escherichia coli
-
Manually annotated by BRENDA team
Shiota, T.; Baugh, C.M.; Jackson, R.; Dillard, R.
The enzymatic synthesis of hydroxymethyldihydropteridine pyrophosphate and dihydrofolate
Biochemistry
8
5022-5028
1969
Lactiplantibacillus plantarum
Manually annotated by BRENDA team
Griffin, M.J.; Brown, G.M.
The biosynthesis of folic acid. III. Enzymatic formation of dihydrofolic acid from dihydropteroic acid and of tetrahydropteroylpolyglutamic acid compounds from tetrahydrofolic acid
J. Biol. Chem.
239
310-316
1964
Priestia megaterium, Saccharomyces cerevisiae, Corynebacterium sp., Escherichia coli, Enterococcus faecalis, Mycobacterium avium, Mycolicibacterium phlei, Neurospora crassa
Manually annotated by BRENDA team
Lacks, S.A.; Greenberg, B.; Lopez, P.
A cluster of four genes encoding enzymes for five steps in the folate biosynthetic pathway of Streptococcus pneumoniae
J. Bacteriol.
177
66-74
1995
Streptococcus pneumoniae
Manually annotated by BRENDA team
Fussenegger, M.; Meyer, T.F.
Cloning and characterization of the Neisseria gonorrhoeae MS11 folC gene
Mol. Gen. Genet.
250
277-285
1996
Neisseria gonorrhoeae
Manually annotated by BRENDA team
Neuburger, M.; Rebeille, F.; Jourdain, A.; Nakamura, S.; Douce, R.
Mitochondia are a major site for folate and thymidylate syntheis in plants
J. Biol. Chem.
271
9466-9472
1996
Pisum sativum
Manually annotated by BRENDA team
Patel, O.; Castelli, L.; Fernley, R.; Coloe, P.; Macreadie, I.
Production of a functional dihydrofolate synthase with a cleavable poly-His tag in Saccharomyces cerevisiae
Biotechnol. Lett.
24
657-662
2002
Saccharomyces cerevisiae, Saccharomyces cerevisiae CUY13
Manually annotated by BRENDA team
Salcedo, E.; Cortese, J.F.; Plowe, C.V.; Sims, P.F.G.; Hyde, J.E.
A bifunctional dihydrofolate synthetase--folylpolyglutamate synthetase in Plasmodium falciparum identified by functional complementation in yeast and bacteria
Mol. Biochem. Parasitol.
112
239-252
2001
Plasmodium falciparum (Q9U738), Plasmodium falciparum
Manually annotated by BRENDA team
Ravanel, S.; Cherest, H.; Jabrin, S.; Grunwald, D.; Surdin-Kerjan, Y.; Douce, R.; Rebeille, F.
Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
98
15360-15365
2001
Arabidopsis thaliana (Q8W041), Arabidopsis thaliana
Manually annotated by BRENDA team
Mathieu, M.; Debousker, G.; Vincent, S.; Viviani, F.; Bamas-Jacques, N.; Mikol, V.
Escherichia coli FolC structure reveals an unexpected dihydrofolate binding site providing an attractive target for anti-microbial therapy
J. Biol. Chem.
280
18916-18922
2005
Escherichia coli
Manually annotated by BRENDA team
Levin, I.; Giladi, M.; Altman-Price, N.; Ortenberg, R.; Mevarech, M.
An alternative pathway for reduced folate biosynthesis in bacteria and halophilic archaea
Mol. Microbiol.
54
1307-1318
2004
Haloarcula marismortui, Halobacterium salinarum, Haloferax volcanii
Manually annotated by BRENDA team
Hauser, P.M.; Macreadie, I.G.
Isolation of the Pneumocystis carinii dihydrofolate synthase gene and functional complementation in Saccharomyces cerevisiae
FEMS Microbiol. Lett.
256
244-250
2006
Pneumocystis carinii (Q2VIK1), Pneumocystis carinii
Manually annotated by BRENDA team
Wang, P.; Wang, Q.; Yang, Y.; Coward, J.K.; Nzila, A.; Sims, P.F.; Hyde, J.E.
Characterisation of the bifunctional dihydrofolate synthase-folylpolyglutamate synthase from Plasmodium falciparum; a potential novel target for antimalarial antifolate inhibition
Mol. Biochem. Parasitol.
172
41-51
2010
Plasmodium falciparum
Manually annotated by BRENDA team
Bacon, D.; Tang, D.; Salas, C.; Roncal, N.; Lucas, C.; Gerena, L.; Tapia, L.; Llanos-Cuentas, A.; Garcia, C.; Solari, L.; Kyle, D.; Magill, A.
Effects of point mutations in Plasmodium falciparum dihydrofolate reductase and dihydropterate synthase genes on clinical outcomes and in vitro susceptibility to sulfadoxine and pyrimethamine
PLoS ONE
4
e6762
2009
Plasmodium falciparum
Manually annotated by BRENDA team
Zhao, F.; Wang, X.D.; Erber, L.N.; Luo, M.; Guo, A.Z.; Yang, S.S.; Gu, J.; Turman, B.J.; Gao, Y.R.; Li, D.F.; Cui, Z.Q.; Zhang, Z.P.; Bi, L.J.; Baughn, A.D.; Zhang, X.E.; Deng, J.Y.
Binding pocket alterations in dihydrofolate synthase confer resistance to para-aminosalicylic acid in clinical isolates of Mycobacterium tuberculosis
Antimicrob. Agents Chemother.
58
1479-1487
2014
Mycobacterium tuberculosis variant bovis (A0A0H3M8P3), Mycobacterium tuberculosis variant bovis, Mycobacterium tuberculosis (I6Y0R5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (I6Y0R5)
Manually annotated by BRENDA team
Luraschi, A.; Cisse, O.H.; Monod, M.; Pagni, M.; Hauser, P.M.
Functional characterization of the Pneumocystis jirovecii potential drug targets dhfs and abz2 involved in folate biosynthesis
Antimicrob. Agents Chemother.
59
2560-2566
2015
Pneumocystis jirovecii (L0PF87), Pneumocystis jirovecii, Pneumocystis jirovecii SE8 (L0PF87)
Manually annotated by BRENDA team
Cheng, V.W.; Leung, K.S.; Kwok, J.S.; Leung, R.K.; Yang, K.Y.; Chan, R.C.; Kam, K.M.; Tsui, S.K.
Phylogenetic and structural significance of dihydrofolate synthase (folC) mutations in drug-resistant Mycobacterium tuberculosis
Microb. Drug Resist.
22
545-551
2016
Mycobacterium tuberculosis (I6Y0R5), Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618 / H37Rv (I6Y0R5)
Manually annotated by BRENDA team