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6.3.2.10: UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase, go to the full flat file.

Word Map on EC 6.3.2.10

Reaction

ATP
+
UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
+
D-alanyl-D-alanine
=
ADP
+
phosphate
+
UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine

Synonyms

AbMurF, ATP-dependent UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase, D-Ala-D-Ala-adding enzyme, More, MurF, MurF1, Synthetase, uridine diphosphoacetylmuramoylpentapeptide, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase, UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase, UDPacetylmuramoylpentapeptide synthetase, Uridine diphosphoacetylmuramoylpentapeptide synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.10 UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase

Specific Activity

Specific Activity on EC 6.3.2.10 - UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.006
-
purified recombinant mutant K202A, in presence of butylamine
0.016
-
purified recombinant mutant K202A
0.032
-
purified recombinant mutant K202A, in presence of propylamine
0.046
-
purified recombinant mutant K202A, in presence of ethylamine
0.052
-
purified recombinant mutant K202A, in presence of formate
0.062
0.437
-
purified recombinant mutant K202A, in presence of butyrate
0.511
-
purified recombinant mutant K202A, in presence of acetate
0.55
-
purified recombinant wild-type enzyme, in presence of methylamine
0.71
-
purified recombinant wild-type enzyme, in presence of propylamine
0.72
-
purified recombinant wild-type enzyme, in presence of butylamine
0.76
-
purified recombinant wild-type enzyme, in presence of propionate
0.769
-
purified recombinant mutant K202A, in presence of propionate
0.8
-
purified recombinant wild-type enzyme, in presence of ethylamine
0.88
-
purified recombinant wild-type enzyme, in presence of acetate
0.93
-
purified recombinant wild-type enzyme, in presence of formate
1.24
-
purified recombinant wild-type enzyme, in presence of butyrate
1.27
-
purified recombinant wild-type enzyme
11.2
purified recombinant wild-type enzyme, 30°C
13.9
purified recombinant wild-type enzyme, 42°C