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6.3.1.9: trypanothione synthase

This is an abbreviated version!
For detailed information about trypanothione synthase, go to the full flat file.

Word Map on EC 6.3.1.9

Reaction

glutathione
+
glutathionylspermidine
 +
ATP
=
N1,N8-bis(glutathionyl)spermidine
 +
ADP
 +
phosphate

Synonyms

Cf-TS, GenBank AF006615-derived protein GI 3004644, LdTryS, LiTRYS, Synthetase, trypanothione, Synthetase, trypanothione (Crithidia fasciculata strain HS6 gene Cf-TS), T(SH)2 synthetase, TbTryS, TcTryS, TRS, trypanothione synthase, Trypanothione synthetase, Trypanothione synthetase (Crithidia fasciculata strain HS6 gene Cf-TS), trypanothione synthetase-amidase, TryS, TSA, TSH synthetase, tyrS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.9 trypanothione synthase

Purification

Purification on EC 6.3.1.9 - trypanothione synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, partially purified
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, and ion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 star (DE3) by nickel affinity chromatography and tag cleavage through thrombin
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage though thrombin, the tag is removed by benzamidine affinity chromatography, the purified enzyme is dialysed
recombinant His-tagged enzyme from Escherichia coli strain Tuner(DE3) by nickel affinity chromatography and tag cleavage by tobacco etch virus protease, followed by a second nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3) or Tuner (DE3) by affinity chromatography
recombinant His-tagged TcTryS, Ni2+-affinity, anion exchange
recombinant His-tagged wild-type enzyme and mutants from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain Tuner (DE3) CodonPlus by nickel affinity chromatography and gel filtration