6.3.1.9: trypanothione synthase

This is an abbreviated version!
For detailed information about trypanothione synthase, go to the full flat file.

Reaction

Synonyms

Cf-TS, GenBank AF006615-derived protein GI 3004644, LdTryS, LiTRYS, Synthetase, trypanothione, Synthetase, trypanothione (Crithidia fasciculata strain HS6 gene Cf-TS), T(SH)2 synthetase, TbTryS, TcTryS, TRS, trypanothione synthase, Trypanothione synthetase, Trypanothione synthetase (Crithidia fasciculata strain HS6 gene Cf-TS), trypanothione synthetase-amidase, TryS, TSA, TSH synthetase, tyrS

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

                6.3.1.9 trypanothione synthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
15	Activating Compound
47	AA Sequence
8	Application
2	CAS Registry Number
16	Cloned(Commentary)
13	Cofactor
2	Crystallization (Commentary)
3	Expression
29	General Information
1	General Stability
104	IC50 Value
174	Inhibitors
16	Ki Value [mM]
41	KM Value [mM]
5	Localization
18	Metals/Ions
9	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
37	Organism
6	Pathway
5	PDB ID
13	pH Optimum
3	pH Range
17	Protein Variants
16	Purification (Commentary)
5	Reaction
1	Reaction Type
36	Reference
18	Source Tissue
5	Specific Activity [micromol/min/mg]
83	Substrates and Products (Substrate)
11	Subunits
68	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
13	Turnover Number [1/s]

Natural Substrates Products

print visible entries

print all entries

Natural Substrates Products on EC 6.3.1.9 - trypanothione synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

6 entries

gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

2 entries

gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

3 entries

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

17 entries

glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

2 entries

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

16 entries

glutathione + spermidine + ATP

N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate

3 entries

N1-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

3 entries

N8-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

3 entries

additional information

?

-

11 entries

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Leishmania major

Q711P7

trypanothione synthase overall reaction

662802

-

-

?

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Leishmania major

-

overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview

728592

-

-

?

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Trypanosoma brucei

-

important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids

661011

-

-

?

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Trypanosoma brucei

-

trypanothione synthase overall reaction, trypanothione metabolism overview

661810

-

-

?

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Trypanosoma brucei

-

overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase

727951

-

-

?

2 glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Trypanosoma brucei 449

-

overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase

727951

-

-

?

gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

Crithidia fasciculata

O60993

synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants

965

-

-

?

gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

Crithidia fasciculata

-

the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione

964

-

-

?

gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

Crithidia luciliae

-

-

650657

-

650657

?

gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

Entamoeba histolytica

-

-

650657

-

650657

?

gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP

N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

Trypanosoma cruzi

Q9GT49

-

652306

trivial name trypanothione

652306

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Crithidia fasciculata

Q5DM89

-

662446

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania donovani

Q8IFU8

-

746270

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania donovani MHOM / IN / 80 / Dd8

Q8IFU8

-

746270

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania donovani MHOM / IN / 83 / Ag

Q8IFU8

-

746270

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania infantum

A4I2Z3

-

744964, 746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania infantum

-

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263

A4I2Z3

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania infantum MHOM / MA / 67 / ITMAP263

A4I2Z3

-

744964

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania major

Q711P7

trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9

662802

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei

-

trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei

661810

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei

-

trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei

661011

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei brucei

Q8IEX1

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei brucei 427

Q8IEX1

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma cruzi

-

-

744910

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma cruzi

Q9GT49

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma cruzi CL Brener

Q9GT49

-

746173

-

-

?

glutathione + glutathionylspermidine + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma cruzi DTU I

-

-

744910

-

-

?

glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Leishmania donovani

G5D5D5

-

727114

-

-

?

glutathione + spermidine + 2 ATP

N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate

Leishmania donovani UR6

G5D5D5

-

727114

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania donovani

Q8IFU8

-

746270

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania donovani MHOM / IN / 80 / Dd8

Q8IFU8

-

746270

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania donovani MHOM / IN / 83 / Ag

Q8IFU8

-

746270

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania infantum

A4I2Z3

-

744964, 746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania infantum

-

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263

A4I2Z3

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania infantum MHOM / MA / 67 / ITMAP263

A4I2Z3

-

744964

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Leishmania major

Q711P7

trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9

662802

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma brucei

-

trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei

661810

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma brucei

-

trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei

661011

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma brucei brucei

Q8IEX1

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma brucei brucei 427

Q8IEX1

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma cruzi

-

-

744910

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma cruzi

Q9GT49

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma cruzi CL Brener

Q9GT49

-

746173

-

-

?

glutathione + spermidine + ATP

glutathionylspermidine + ADP + phosphate

Trypanosoma cruzi DTU I

-

-

744910

-

-

?

glutathione + spermidine + ATP

N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate

Leishmania major

-

reaction of EC 6.3.1.8, step one

728592

-

-

?

glutathione + spermidine + ATP

N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate

Trypanosoma brucei

-

reaction of EC 6.3.1.8, step one

727951

-

-

?

glutathione + spermidine + ATP

N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate

Trypanosoma brucei 449

-

reaction of EC 6.3.1.8, step one

727951

-

-

?

N1-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania major

-

step two

728592

-

-

?

N1-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei

-

step two

727951

-

-

?

N1-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei 449

-

step two

727951

-

-

?

N8-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Leishmania major

-

step two, N8-glutathionylspermidine is the favoured substrate

728592

-

-

?

N8-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei

-

step two

727951

-

-

?

N8-glutathionylspermidine + glutathione + ATP

N1,N8-bis(glutathionyl)spermidine + ADP + phosphate

Trypanosoma brucei 449

-

step two

727951

-

-

?

additional information

?

-

Crithidia fasciculata

Q5DM89

the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8

662446

-

-

?

additional information

?

-

Crithidia fasciculata

-

the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8

662446

-

-

?

additional information

?

-

Leishmania donovani

G5D5D5

trypanothione synthetase synthesizes trypanothione from glutathione and spermidine

727114

-

-

?

additional information

?

-

Leishmania donovani

-

trypanothione synthetase synthesizes trypanothione from glutathione and spermidine

727114

-

-

?

additional information

?

-

Leishmania donovani UR6

G5D5D5

trypanothione synthetase synthesizes trypanothione from glutathione and spermidine

727114

-

-

?

additional information

?

-

Leishmania major

Q711P7

phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome

662802

-

-

?

additional information

?

-

Leishmania major

-

phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome

662802

-

-

?

additional information

?

-

Leishmania major

Q711P7

the bifunctional trypanothione synthetase-amidase catalyzes biosynthesis and hydrolysis of the glutathione-spermidine adduct trypanothione, the principal intracellular thiolredox metabolite in parasitic trypanosomatids

693074

-

-

?

additional information

?

-

Trypanosoma brucei

-

the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction

661810

-

-

?

additional information

?

-

Trypanosoma brucei

-

in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site

727951

-

-

?

additional information

?

-

Trypanosoma brucei 449

-

in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site

727951

-

-

?