6.3.1.9: trypanothione synthase
This is an abbreviated version!
For detailed information about trypanothione synthase, go to the full flat file.
Word Map on EC 6.3.1.9
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6.3.1.9
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trypanosoma
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leishmania
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trypanosomatids
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brucei
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cruzi
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fasciculata
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crithidia
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n1,n8-bisglutathionylspermidine
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chagas
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infantum
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tryparedoxine
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antileishmanial
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parasite-specific
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kinetoplastida
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antitrypanosomal
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drug development
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synthesis
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pharmacology
- 6.3.1.9
- trypanosoma
- leishmania
-
trypanosomatids
- brucei
- cruzi
- fasciculata
- crithidia
-
n1,n8-bisglutathionylspermidine
-
chagas
- infantum
-
tryparedoxine
-
antileishmanial
-
parasite-specific
- kinetoplastida
-
antitrypanosomal
- drug development
- synthesis
- pharmacology
Reaction
Synonyms
Cf-TS, GenBank AF006615-derived protein GI 3004644, LdTryS, LiTRYS, Synthetase, trypanothione, Synthetase, trypanothione (Crithidia fasciculata strain HS6 gene Cf-TS), T(SH)2 synthetase, TbTryS, TcTryS, TRS, trypanothione synthase, Trypanothione synthetase, Trypanothione synthetase (Crithidia fasciculata strain HS6 gene Cf-TS), trypanothione synthetase-amidase, TryS, TSA, TSH synthetase, tyrS
ECTree
6.3.1.9 trypanothione synthaseAdvanced search results
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Engineering on EC 6.3.1.9 - trypanothione synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C59A
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mutant lacks the amidase activity, while the synthetase activity is similar to wild-type. Constructed for the production of trypanothione and trypanothione disulfide in >200 mg quantities. The protocol also allows the synthesis of related glutathione conjugates
C57A
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functional synthetase devoid of amidase activity. In the presence of inducer, this line showed decreased growth in vitro and decreased virulence in vivo, indicating that the amidase function is not absolutely required for viability
additional information
additional information
elimination of both LiTRYS alleles is not possible unless parasites are previously complemented with an episomal copy of the gene. Retention of extrachromosomal LiTRYS in the trys-/-/+TRYS line after several passages in culture
additional information
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elimination of both LiTRYS alleles is not possible unless parasites are previously complemented with an episomal copy of the gene. Retention of extrachromosomal LiTRYS in the trys-/-/+TRYS line after several passages in culture
additional information
Leishmania infantum MHOM / MA / 67 / ITMAP263
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elimination of both LiTRYS alleles is not possible unless parasites are previously complemented with an episomal copy of the gene. Retention of extrachromosomal LiTRYS in the trys-/-/+TRYS line after several passages in culture
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additional information
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enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview
additional information
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enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview
additional information
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introduction of the trypanothione synthetase and reductase genes from Trypanosoma cruzi into Escherichia coli results in decreased intracellular levels of reactive oxygen species. Escherichia coli genomic DNA is protected from radiation-induced DNA damage by 4.6fold in the presence of trypanothione compared to control bacteria. The transgenic Escherichia coli expressing trypanothione are 4.3fold more resistant to killing by 137Cs gamma-radiation
