6.3.1.14: diphthine-ammonia ligase
This is an abbreviated version!
For detailed information about diphthine-ammonia ligase, go to the full flat file.
Reaction
Synonyms
Diphthamide synthase, diphthamide synthetase, DPH6, EC 6.3.2.22, Synthetase, diphthamide, YLR143w
ECTree
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Substrates Products
Substrates Products on EC 6.3.1.14 - diphthine-ammonia ligase
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REACTION DIAGRAM
ATP + diphthine-[peptide 686-VNILDVTLHADAIHR-700] + NH3
AMP + diphosphate + diphthamide-[peptide 686-VNILDVTLHADAIHR-700]
eEF2-derived peptide sequence
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?
ATP + diphthine-[translation elongation factor 2] + NH3
AMP + diphosphate + diphthamide-[translation elongation factor 2]
AMP + diphosphate + diphthamide-[translation elongation factor 2]
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?
ATP + diphthine-[translation elongation factor 2] + NH3
AMP + diphosphate + diphthamide-[translation elongation factor 2]
modified histidine in eEF2 (His699) is located in the tryptic peptide 686-VNILDVTLHADAIHR-700
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ATP + diphthine-[translation elongation factor 2] + NH3
AMP + diphosphate + diphthamide-[translation elongation factor 2]
Saccharomyces cerevisiae ATCC 204508 / S288c
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CDP + phosphate + diphthamide
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11% of activity compared to ATP
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CTP + diphthine + ammonia
CDP + phosphate + diphthamide
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11% of activity compared to ATP
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?
GDP + phosphate + diphthamide
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14% of activity compared to ATP
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?
GTP + diphthine + ammonia
GDP + phosphate + diphthamide
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14% of activity compared to ATP
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?
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the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide
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additional information
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Saccharomyces cerevisiae ATCC 204508 / S288c
the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide
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?