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6.2.1.4: succinate-CoA ligase (GDP-forming)

This is an abbreviated version!
For detailed information about succinate-CoA ligase (GDP-forming), go to the full flat file.

Word Map on EC 6.2.1.4

Reaction

GTP
+
succinate
+
CoA
=
GDP
+
phosphate
+
succinyl-CoA

Synonyms

ATP-specific SCS, G-SCS, G-STK, G-SUCL, GDP-forming SUCL, GTP-dependent succinate:CoA ligase, GTP-forming SUCL, GTP-specific succinate:CoA ligase, GTP-specific succinyl-CoA synthetase, GTP-specific SUCL, GTPSCS, SCS, SCS-alpha, SCS-beta, SCS-betaG, ScsA, SCSalpha, SucCDAm, Succinate thiokinase, succinate-CoA ligase, Succinic thiokinase, Succinyl CoA synthetase, Succinyl coenzyme A synthetase, Succinyl coenzyme A synthetase (GDP-forming), Succinyl coenzyme A synthetase (guanosine diphosphate-forming), succinyl-CoA synthase, Succinyl-CoA synthetase, Succinyl-CoA synthetase (GDP-forming), succinyl-CoA synthetase subunit beta, succinyl-coenzyme A synthetase, SUCL, SUCLG1, SUCLG2, Synthetase, succinyl coenzyme A (guanosine diphosphate-forming)

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.4 succinate-CoA ligase (GDP-forming)

Crystallization

Crystallization on EC 6.2.1.4 - succinate-CoA ligase (GDP-forming)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour diffusion method using a precipitant solution containing 10% (v/v) isopropanol, 100 mM ammonium sulfate, 20% (w/v) polyethylene glycol 4000, and 100 mM HEPES or Bicine, pH 7.5
-
in complex with CoA, hanging drop vapor diffusion method, using 1.6 M ammonium sulfate, 1 mM 2-mercaptoethanol, 50 mM sodium/potassium phosphate pH 7.0
in complex with succinate, magnesium ions and CoA, hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 and 0.2 M magnesium formate
hanging drop vapour diffusion method using using 10% polyethylene glycol 3350, 100 mM 4-morpholinoethanesulfonic acid (pH 6.4 for 1 M solution) and 200 mM KCl and a protein solution containing 5 mM GDP and 10 mM MnCl2
-
purified enzyme in complex with GDP-Mn2+, protein in 50 mM KCl, 0.1 mM EDTA, 50 mM Tris-HCl, pH 7.4, X-ray diffraction structure determination and analysis at 2.35 A resolution
-