6.2.1.12: 4-coumarate-CoA ligase

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For detailed information about 4-coumarate-CoA ligase, go to the full flat file.

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Synonyms

4-CL, 4-coumarate CoA ligase, 4-coumarate coenzyme A ligase, 4-coumarate/coenzyme A ligase, 4-coumarate: CoA ligase, 4-coumarate: coenzyme A ligase, 4-coumarate:CoA ligase, 4-coumarate:CoA-ligase, 4-coumarate:coenzyme A (CoA) ligase, 4-Coumarate:coenzyme A ligase, 4-coumarate:coenzyme-A ligase-1, 4-coumaroyl CoA ligase, 4-coumaroyl coenzyme A ligase, 4-coumaroyl-CoA ligase, 4-coumaroyl-CoA synthase, 4-coumaroyl:CoA ligase, 4-Coumaryl-CoA synthetase, 4CL, 4CL-A1, 4CL-A2, 4CL-B, 4CL1, 4CL10, 4CL2, 4CL3, 4CL4, 4CL5, 4CL7, Ar4CL, At4CL1, Caffeolyl coenzyme A synthetase, caffeoyl-CoA ligase, caffeoyl-CoA synthetase, caffeoyl-coenzyme A synthetase, CarB, Clone 4CL14, Clone 4CL16, coumarate:CoA ligase, Feruloyl CoA ligase, Feruloyl coenzyme A synthetase, Hc4CL, Hydroxy-cinnamate:CoA ligase, Hydroxycinnamate:CoA ligase, Hydroxycinnamoyl CoA synthetase, LJ4CL1, OB4CL_ctg4, OS4CL, Os4Cl1, Os4Cl2, Os4Cl3, Os4Cl4, Os4Cl5, p-Coumaroyl CoA ligase, p-coumaroyl-CoA ligase, p-Coumaryl coenzyme A synthetase, p-Coumaryl-CoA ligase, p-Coumaryl-CoA synthetase, p-Hydroxycinnamic acid:CoA ligase, p-Hydroxycinnamoyl coenzyme A synthetase, Pc4CL2, Pl4CL1, Pl4CL2, Sa4CL1, Sa4CL2, Sa4CL3, Sinapoyl coenzyme A snthetase, Synthetase, p-coumaroyl coenzyme A, type I 4CL

ECTree

     6 Ligases

         6.2 Forming carbon-sulfur bonds

             6.2.1 Acid-thiol ligases

                6.2.1.12 4-coumarate-CoA ligase

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Results	in table
2	Activating Compound
2180	AA Sequence
8	Application
1	CAS Registry Number
62	Cloned(Commentary)
38	Cofactor
2	Crystallization (Commentary)
17	Expression
42	General Information
4	General Stability
2	IC50 Value
47	Inhibitors
68	kcat/KM [mM/s]
4	Ki Value [mM]
279	KM Value [mM]
6	Localization
37	Metals/Ions
26	Molecular Weight [Da]
65	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
145	Organism
28	Pathway
17	PDB ID
26	pH Optimum
5	pH Range
1	pH Stability
5	pI Value
86	Protein Variants
32	Purification (Commentary)
2	Reaction
1	Reaction Type
124	Reference
136	Source Tissue
35	Specific Activity [micromol/min/mg]
9	Storage Stability
390	Substrates and Products (Substrate)
16	Subunits
218	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
105	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.2.1.12 - 4-coumarate-CoA ligase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 10 mM sodium/potassium phosphate, pH 7.0, 42 mM mercaptoethanol, 20% ethyleneglycol, stable for several months

Picea abies

-

614

-20°C, 10% or 30% glycerol, or 10% ethylene glycol, stable for 10 days

Erythrina crista-galli

-

616

-20°C, 50% glycerol

Forsythia suspensa

-

607

-20°C, several weeks without significant loss of activity

Glycine max

-

606

-20°C, stable for 2 or 3 months, crude enzyme extract

Populus sp.

-

596

1°C, after two days decrease in enzyme activity

Fragaria x ananassa

-

704148

complete loss of activity in crude enzyme extract after 4 days at 4-6°C. 0.92 M sucrose can reduce the loss of activity to 20%. Complete stabilization in presence of 30% glycerol or 30% ethylene glycol

Petroselinum crispum

-

611

crude enzyme preparation is unstable in buffered aqueous solution at temperatures at or above 0°C

Glycine max

-

608

stable for several weeks at -20°C or two days at 0°C, in 0.1 M Tris-HCl buffer, pH 7.5, 30% glycerol or 15% ethylene glycol

Populus x canadensis

-

615