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6.2.1.12: 4-coumarate-CoA ligase

This is an abbreviated version!
For detailed information about 4-coumarate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.12

Reaction

ATP
+
4-coumarate
 +
CoA
=
AMP
 +
diphosphate
 +
4-coumaroyl-CoA

Synonyms

4-CL, 4-coumarate CoA ligase, 4-coumarate coenzyme A ligase, 4-coumarate/coenzyme A ligase, 4-coumarate: CoA ligase, 4-coumarate: coenzyme A ligase, 4-coumarate:CoA ligase, 4-coumarate:CoA-ligase, 4-coumarate:coenzyme A (CoA) ligase, 4-Coumarate:coenzyme A ligase, 4-coumarate:coenzyme-A ligase-1, 4-coumaroyl CoA ligase, 4-coumaroyl coenzyme A ligase, 4-coumaroyl-CoA ligase, 4-coumaroyl-CoA synthase, 4-coumaroyl:CoA ligase, 4-Coumaryl-CoA synthetase, 4CL, 4CL-A1, 4CL-A2, 4CL-B, 4CL1, 4CL10, 4CL2, 4CL3, 4CL4, 4CL5, 4CL7, Ar4CL, At4CL1, Caffeolyl coenzyme A synthetase, caffeoyl-CoA ligase, caffeoyl-CoA synthetase, caffeoyl-coenzyme A synthetase, CarB, Clone 4CL14, Clone 4CL16, coumarate:CoA ligase, Feruloyl CoA ligase, Feruloyl coenzyme A synthetase, Hc4CL, Hydroxy-cinnamate:CoA ligase, Hydroxycinnamate:CoA ligase, Hydroxycinnamoyl CoA synthetase, LJ4CL1, OB4CL_ctg4, OS4CL, Os4Cl1, Os4Cl2, Os4Cl3, Os4Cl4, Os4Cl5, p-Coumaroyl CoA ligase, p-coumaroyl-CoA ligase, p-Coumaryl coenzyme A synthetase, p-Coumaryl-CoA ligase, p-Coumaryl-CoA synthetase, p-Hydroxycinnamic acid:CoA ligase, p-Hydroxycinnamoyl coenzyme A synthetase, Pc4CL2, Pl4CL1, Pl4CL2, Sa4CL1, Sa4CL2, Sa4CL3, Sinapoyl coenzyme A snthetase, Synthetase, p-coumaroyl coenzyme A, type I 4CL

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.12 4-coumarate-CoA ligase

Cloned

Cloned on EC 6.2.1.12 - 4-coumarate-CoA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
60-66% sequence identity with 4-coumarate:CoA ligase sequences from herbaceous angiosperms
-
a binary vector containing Pto4CL1p promoter fused with 4CL1 gene is transferred into tobacco. The activity of the 4CL1 enzyme doubles in the stems of transgenic tobacco but does not increase in the leaves. The content of lignin is increased 25% in the stem but there is no increase in the leaves of transgenic tobacco
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cloning of 4CL, encoded by a family of four genes 4CL1-4CL4
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DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pET28a, subcloning in Escherichia coli strain M15
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pQE30, subcloning in Escherichia coli strain M15
DNA and amino acid sequence determination and analysis, sequence comparisons
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, quantitative real-time PCR expression analysis
DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of OS4CL-GFP-fusion protein in Arabidopsis thaliana protoplast cytosol, expression of N-terminally His6-tagged OS4CL in Escherichia coli
expressed as fusion protein with stilbene synthase in Saccharomyces cerevisiae and Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli as His-tag fusion protein
expressed in Escherichia coli as His-tag fusion protein and in native form
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15 as His-tag fusion protein
-
expressed in Escherichia coli M15[pRP4] as His-tag fusion protein
-
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
expressed in Escherichia coli strains DH5alpha and M15
-
expressed in Escherichia coli strains JM109 and BLR(DE3)
-
expressed in Nicotiana tabacum roots
expressed in Saccharomyces cerevisiae
expressed in Sf9 cells using the baculovirus system
-
expression in Escherichia coli as His-tag fusion protein
-
expression in Escherichia coli M15(pRP4)
expression in Lactococcus lactis
-
expression in Saccharomyces cerevisiae and Escherichia coli strain BL21, co-expression with stilbene synthase, gene STS, from Vitis vinifera establishing an resveratrol expression system with 4-coumaric acid as precursor, biosynthetic pathway of resveratrol, overview
expression in together with tyramine N-hydroxycinnamoyltransferase in Escherichia coli
-
expression of GST-tagged isozyme Sa4CL1 in Escherichia coli
expression of His-tagged wild-type and mutant enzymes in Escherichia coli, expression of wild-type and mutants in Saccharomyces cerevisiae expressing also the CHS gene
-
expression of His6-tagged isozyme Sa4CL2 in Escherichia coli
expression of His6-tagged isozyme Sa4CL3 in Escherichia coli
expression of isozyme At4CL1, and of the engineered fusion protein of Arabidopsis thaliana At4CL1 and Vitis vinifera stilbene synthase, VvSTS
gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105
-
gene Os4CL1, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis
gene Os4CL2, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis
gene Os4CL3, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis
gene Os4CL4, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis
gene Os4CL5, DNA and amino acid sequence determination and analysis, gene structure and phylogenetic analysis
gene R4CL, expression in Escherichia coli strain BL21
insertion of phenylalanine ammonia lyase from the red yeast Rhodosporidium toruloides, 4-coumarate:coenzyme A ligase from the plant Arabidopsis thaliana, and chalcone synthase from the plant Hypericum androsaemum into a yeast expression vector. Each gene is under the control of its own galactose-inducible promoter. Yeast harboring this vector produces naringenin and pinocembrin through the phenylpropanoid pathway, as well as four by-products, two of which are identified as phloretin and 2',4',6'-trihydroxydihydrochalcone through a sequential side reaction
isozyme Os4Cl1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21
isozyme Os4Cl2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21
isozyme Os4Cl3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21
isozyme Os4Cl4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21
isozyme Os4Cl5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21
nucleotide sequence of cDNA
-
overexpression in Escherichia coli
transgenic Arabidopsis plants containing the At4CL1 or At4CL2 promoter fused to the beta-glucuronidase reporter gene show developmentally regulated GUS expression in the xylem tissues of the root and shoot, identification of regulatory genes involved in the developmental regulation of At4CL, overview
-
two distinct 4CL genes, highly homologous for several hundred base pairs
-