6.1.1.9: valine-tRNA ligase
This is an abbreviated version!
For detailed information about valine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.9
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6.1.1.9
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synthetases
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aminoacylation
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aminoacyl-trna
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valylation
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isoleucyl-trna
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anticodon
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ilers
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turnip
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noncognate
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leucyl-trna
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mischarged
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trna-like
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isoleucyl
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misacylated
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misactivated
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post-transfer
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l-shaped
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aarss
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phenylalanyl-trna
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threonylation
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trnaile
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cysrs
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atp-ppi
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medicine
- 6.1.1.9
- synthetases
- aminoacylation
- aminoacyl-trna
-
valylation
-
isoleucyl-trna
-
anticodon
- ilers
-
turnip
-
noncognate
- leucyl-trna
-
mischarged
-
trna-like
-
isoleucyl
-
misacylated
-
misactivated
-
post-transfer
-
l-shaped
-
aarss
- phenylalanyl-trna
-
threonylation
- trnaile
- cysrs
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atp-ppi
- medicine
Reaction
Synonyms
G7a, glp-4, mitochondrial valyl tRNA synthetase, More, MTTV, Os03g0694900, OsValRS2, Synthetase, valyl-transfer ribonucleate, Val-tRNA synthetase, Valine transfer ribonucleate ligase, Valine translase, Valine--tRNA ligase, ValRS, ValRS1, ValRS2, valS, valyl aminoacyl tRNA synthetase, Valyl transfer ribonucleic acid synthetase, Valyl-transfer ribonucleate synthetase, Valyl-transfer RNA synthetase, Valyl-tRNA ligase, Valyl-tRNA synthetase, valyl-tRNAsynthetase, VARS, VARS-2, Vas1, Vas2, white panicle1
ECTree
6.1.1.9 valine-tRNA ligaseAdvanced search results
results (
results (KM Value
KM Value on EC 6.1.1.9 - valine-tRNA ligase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.3
L-threonine
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ATP-diphosphate exchange reaction, native wild-type enzyme, pH 7.5, 25°C
0.00061
tRNAVal(GAC)
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in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C
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0.00208
tRNAVal(UAC)
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in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C
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0.00108
tRNAVal(UAC-2)
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in 100 mM HEPES (pH 7.2), 20 mM KCl, 30 mM MgCl2, at 30°C
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0.11
ATP
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ATP-diphosphate exchange reaction, His-tagged wild-type and mutant enzymes, pH 7.5, 25°C
0.0019
L-valine
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aminoacylation reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C
0.002
L-valine
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with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C
0.0048
L-valine
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with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C
0.005
L-valine
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with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C
0.0088
L-valine
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with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C
0.047
L-valine
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aminoacylation reaction, His-tagged wild-type enzyme, pH 7.5, 25°C
0.062
L-valine
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ATP-diphosphate exchange reaction, native wild-type enzyme, pH 7.5, 25°C
0.07
L-valine
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ATP-diphosphate exchange reaction, His-tagged wild-type enzyme, pH 7.5, 25°C
0.072
L-valine
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ATP-diphosphate exchange reaction, His-tagged K277A mutant enzyme, pH 7.5, 25°C
0.00008
tRNAVal
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aminoacylation reaction with L-valine, wild-type enzyme, pH 7.5, 25°C
0.0001
tRNAVal
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aminoacylation reaction with L-valine, His-tagged wild-type enzyme, pH 7.5, 25°C
0.0001
tRNAVal
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aminoacylation reaction with L-valine, K277A mutant enzyme, pH 7.5, 25°C
0.00026
tRNAVal
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aminoacylation reaction with L-valine, His-tagged K277A mutant enzyme, pH 7.5, 25°C
0.00165
tRNAVal
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substrate from mitochondria, , mitochondrial and cytoplasmic enzyme
additional information
additional information
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Km-values of ATP analogs
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additional information
additional information
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Km of anticodon loop mutants of turnip yellow mosaic virus RNA
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additional information
additional information
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Km for tRNAVal mutants, loss of the electrostatically interactions between the coiled-coil domain of the enzyme and base A20 and tertiary bas pair G19.C56 increases the Km for the tRNA by 28fold
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additional information
additional information
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steady-state parameters for tRNA-independent pre-transfer editing by ValRS and its mutants determined by varying concentrations of noncognate threonine, overview
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