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0.0035 - 6.08
Borellia burgdorferi tRNALys
-
0.0025 - 0.0035
Escherichia coli G2.U71 tRNA
-
0.004
Escherichia coli tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
0.002
Escherichia coli tRNALys CNBr-treated
-
recombinant enzyme, pH 7.2, 37°C
-
0.004 - 0.055
Escherichia coli wild type tRNALys
-
6.2 - 7.8
human tRNALys3
-
0.00067
L-arginyl-tRNALys
-
deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
14.5
L-Lysine amide
-
forward reaction, pH 8.0, 30°C
221
L-Lysine hydroxamate
-
forward reaction, pH 8.0, 30°C
0.00023 - 0.0088
L-lysyl-tRNALys
0.00056
L-methionyl-tRNALys
-
deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
-
0.00076
L-threonyl-tRNALys
-
deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
-
0.2
Methanococcus maripaludis tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
0.05
Methanococcus maripaludis tRNALys CNBr-treated
-
recombinant enzyme, pH 7.2, 37°C
-
0.00026 - 6.08
tRNALys,3'-59mer
-
0.014 - 0.062
tRNALysCUU
-
0.0095 - 0.043
tRNALysGUU
-
0.0079
tRNALysU35C
-
human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
-
0.0068
tRNALysU36A
-
human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
-
0.046
tRNALysU36C
-
human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
-
0.00012 - 0.68
tRNALysUUU
-
0.12
tRNALysUUUmodified
-
-
-
6.3 - 7.1
yeast tRNALys
-
additional information
additional information
-
0.00051
ATP
-
mutant enzyme Y269S
0.0011
ATP
-
mutant enzyme T31G
0.013
ATP
-
pH 7.2, 37°C, mutant enzyme E240Q
0.017
ATP
-
pH 7.2, 37°C, mutant enzyme E278Q
0.017
ATP
-
pH 7.2, 37°C, mutant enzyme E428Q
0.017
ATP
-
mutant enzyme Y269F
0.054
ATP
-
pH 7.2, 37°C, mutant enzyme E278D
0.14
ATP
-
pH 7.2, 37°C, mutant enzyme E240D
0.17
ATP
-
pH 7.2, 37°C, mutant enzyme Y280F
0.3
ATP
-
ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C
0.35
ATP
-
pH 7.2, 37°C, mutant enzyme Y280S
0.57
ATP
-
wild type enzyme
0.67
ATP
-
pH 7.2, 37°C, mutant enzyme F426H
0.8
ATP
-
pH 7.2, 37°C, mutant enzyme G216A
1
ATP
-
pH 7.2, 37°C, mutant enzyme E428D
1
ATP
-
pH 7.2, 37°C, mutant enzyme N424D
1.7
ATP
-
mutant enzyme T31S
2.2
ATP
-
pH 7.2, 37°C, mutant enzyme N424Q
2.4
ATP
-
pH 7.2, 37°C, mutant enzyme F426W
3.4
ATP
-
pH 7.2, 37°C, wild-type enzyme
5
ATP
-
recombinant wild-type enzyme, pH 7.5, 25°C
5.1
ATP
-
recombinant N-terminally truncated enzyme, pH 7.5, 25°C
34.7
ATP
-
ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C
36.4
ATP
-
ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C
37.3
ATP
-
ATP-diphosphate exchange reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C
38.6
ATP
-
ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C
40.9
ATP
-
ATP-diphosphate exchange
42.8
ATP
-
ATP-diphosphate exchange reaction, recombinant wild-type enzyme, pH 8.0, 37°C
0.0035
Borellia burgdorferi tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
0.79
Borellia burgdorferi tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
6.08
Borellia burgdorferi tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
0.0025
Escherichia coli G2.U71 tRNA
-
recombinant enzyme, pH 7.2, 37°C
-
0.0035
Escherichia coli G2.U71 tRNA
-
recombinant enzyme, pH 7.2, 37°C
-
0.004
Escherichia coli wild type tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
0.055
Escherichia coli wild type tRNALys
-
recombinant enzyme, pH 7.2, 37°C
-
6.2
human tRNALys3
-
recombinant wild-type enzyme, pH 7.5, 25°C
-
7.8
human tRNALys3
-
recombinant N-terminally truncated enzyme, pH 7.5, 25°C
-
0.00012
L-lysine
-
mutant enzyme H242A
0.00017
L-lysine
-
mutant enzyme W220L
0.00065
L-lysine
-
L-lysine lactam formation, pH 7.4, 37°C
0.00075
L-lysine
-
mutant enzyme W220A
0.0026
L-lysine
-
mutant enzyme Y269S
0.0046
L-lysine
-
mutant enzyme T31G
0.0087
L-lysine
-
pH 7.2, 37°C, mutant enzyme E240Q
0.0143
L-lysine
-
at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin
0.015
L-lysine
-
pH 7.2, 37°C, mutant enzyme E278Q
0.02
L-lysine
-
pH 7.2, 37°C, mutant enzyme E428D
0.031
L-lysine
-
pH 7.2, 37°C, mutant enzyme Y280F
0.086
L-lysine
-
pH 7.2, 37°C, mutant enzyme E240D
0.099
L-lysine
-
mutant enzyme Y269F
0.13
L-lysine
-
mutant enzyme W220Y
0.16
L-lysine
-
pH 7.2, 37°C, mutant enzyme E278D
0.29
L-lysine
-
mutant enzyme H242L
0.34
L-lysine
-
wild type enzyme
0.45
L-lysine
-
mutant enzyme G29A
0.53
L-lysine
-
mutant enzyme T31S
0.6
L-lysine
-
pH 7.2, 37°C, mutant enzyme F426W
0.65
L-lysine
-
pH 7.2, 37°C, mutant enzyme N424Q
1
L-lysine
-
pH 7.2, 37°C, mutant enzyme E428D
1.6
L-lysine
-
pH 7.2, 37°C, mutant enzyme N424D
1.6
L-lysine
-
pH 7.2, 37°C, mutant enzyme Y280S
1.8
L-lysine
-
pH 7.2, 37°C, wild-type enzyme
2.4
L-lysine
-
pH 7.2, 37°C, mutant enzyme F426H
3 - 6
L-lysine
-
aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C
3.13
L-lysine
-
aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C
3.13
L-lysine
-
aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C
3.2
L-lysine
native enzyme, pH 8.0, 30°C
3.23
L-lysine
-
aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C
3.3
L-lysine
-
aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C
3.4
L-lysine
-
pH 7.2, 37°C, mutant enzyme G216A
3.53
L-lysine
-
aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C
3.58
L-lysine
-
aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C
3.6
L-lysine
recombinant enzyme without t7-tag
4.1
L-lysine
recombinant T7-tagged enzyme, pH 8.0, 30°C
5.1
L-lysine
-
recombinant N-terminally truncated enzyme, pH 7.5, 25°C
5.8
L-lysine
-
recombinant wild-type enzyme, pH 7.5, 25°C
8.2
L-lysine
-
mutant enzyme G469A, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
41.7
L-lysine
-
wild type enzyme, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
45.7
L-lysine
-
forward reaction, pH 8.0, 30°C
85
L-lysine
-
mutant enzyme A233S, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
0.00023
L-lysyl-tRNALys
-
deacylation reaction, pH 7.4, 37°C, in absence of DTT
0.0088
L-lysyl-tRNALys
-
deacylation reaction, pH 7.4, 37°C, in presence of 50 mM DTT
0.34
tRNALys
-
wild-type tRNALys
0.38
tRNALys
-
human wild-type tRNALys, 30°C, N-terminally truncated mutant enzyme
1.7
tRNALys
-
human wild-type tRNALys, 20°C, wild-type enzyme
2
tRNALys
-
human wild-type tRNALys, 30°C, wild-type enzyme
2.7
tRNALys
-
18S synthetase complex
5.5
tRNALys
-
free enzyme
0.00026
tRNALys,3'-59mer
-
human tRNALys mutant + human tRNALys,3'-7mer mutant, 20°C, wild-type enzyme
-
0.88
tRNALys,3'-59mer
-
human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme
-
1.5
tRNALys,3'-59mer
-
human tRNALys mutant + human tRNALys,3'-17mer mutant, 20°C, wild-type enzyme
-
6.08
tRNALys,3'-59mer
-
human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme
-
0.014
tRNALysCUU
-
-
-
0.062
tRNALysCUU
-
wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
-
0.0095
tRNALysGUU
-
-
-
0.043
tRNALysGUU
-
wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
-
0.00012
tRNALysUUU
-
mutant enzyme W220L
-
0.00013
tRNALysUUU
-
mutant enzyme H242A
-
0.00056
tRNALysUUU
-
mutant enzyme W220A
-
0.0023
tRNALysUUU
-
mutant enzyme Y269S
-
0.005
tRNALysUUU
-
mutant enzyme T31G
-
0.14
tRNALysUUU
-
mutant enzyme Y269F
-
0.23
tRNALysUUU
-
mutant enzyme W220Y
-
0.29
tRNALysUUU
-
mutant enzyme H242L
-
0.33
tRNALysUUU
-
wild type enzyme
-
0.44
tRNALysUUU
-
wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
-
0.59
tRNALysUUU
-
mutant enzyme G29A
-
0.68
tRNALysUUU
-
mutant enzyme T31S
-
6.3
yeast tRNALys
-
recombinant wild-type enzyme, pH 7.5, 25°C
-
7.1
yeast tRNALys
-
recombinant N-terminally truncated enzyme, pH 7.5, 25°C
-
additional information
additional information
-
hyperbolic dependence of kapp on the initial ATP concentration
-
additional information
additional information
-
turnover numbers of tRNALys variants
-